Macedonian Veterinary Review

Original Scientific Article

Effect of sperm extender and dilution ratio on the sperm motility, fertility, and hatching rates of depik fish Rasbora tawarensis (Pisces: Cyprinidae) eggs

Zainal Abidin Muchlisin *

Dhea Febby Aldila ,

Putri Inten Saraх ,

Kartini Eriani

Iwan Hasri

Nur Fadli

Abdullah Abbas Muhammadar

Luvi Syafrida Handayani ,

Siti Maulida ,

Mohd Nor Siti-Azizah

Filiz Kutluyer Kocabas

Mehmet Kocabas

Mac Vet Rev 2024; 47 (2): 123 - 129

10.2478/macvetrev-2024-0022

Received: 25 February 2024

Received in revised form: 05 August 2024

Accepted: 06 August 2024

Available Online First: 23 August 2024

Published on: 15 October 2024

Correspondence: Zainal Abidin Muchlisin, muchlisinza@usk.ac.id

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Abstract

There is no known artificial breeding method for depik fish Rasbora tawarensis. Sperm extender and the dilution ratio are crucial factors in the artificial breeding of fish. The current study aimed to assess the effect of several common fish sperm extenders and its dilution ratio on sperm characteristics and fertilization of depik fish eggs. Two series of experiments were performed to find the best extender and its dilution ratio. In the first experiment, five types of extenders were tested: Ringer’s solution, physiological solution, coconut water, sugarcane water, and roomie water. The second experiment studied four dilution levels of sperm and extender (v/v): 1:20, 1:30, 1:40, and 1:50. Every treatment was conducted in four replicates and the experiments were conducted consequently so that the findings in the first experiment were applied in the second. The first experiment showed that Ringer’s is the best extender for depik sperm with sperm motility, fertilization and hatching rates of 71.00%, 69.30%, and 53.66%, respectively; and the second experiment revealed that Ringer’s at dilution ratio 1:40 gave higher sperm motility, fertilization, and hatching rates of 74.66, 70.33, and 59.00%, respectively. In conclusion, Ringer’s solution was the most favorable extender for sperm which yielded best fertilization results at 1:40 dilution ratio (sperm:extender, v/v) in depik fish.

Keywords: depik fish, sperm, fertilization, Ringer’s solution, physiological solution

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Copyright

© 2024 Muchlisin Z.A. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Conflict of Interest Statement

The authors declared that they have no potential lict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 47, Issue 2, Pages 123-129, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2024-0022