Original Scientific Article
Short Synch - a reproductive management tool to increase the reproductive performance in dairy cows
Emilija Murdzeva ,
Martin Nikolovski ,
Stevan Tokin ,
Ljupco Mickov ,
Ksenija Ilievska ,
Zoran Dimitrovski ,
Igor Esmerov ,
Toni Dovenski ,
Branko Atanasov *

Mac Vet Rev 2025; 48 (1): i - vii

10.2478/macvetrev-2025-0013

Received: 13 November 2024

Received in revised form: 17 December 2024

Accepted: 17 January 2025

Available Online First: 28 January 2025

Published on: 15 March 2025

Correspondence: Branko Atanasov, batanasov@fvm.ukim.edu.mk
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Abstract

Poor estrus detection is the greatest limiting factor for reproductive performance in dairy cows. The study aimed to compare the reproductive performance by assessing the fertility rate (FR) between cows inseminated after detected estrus and those inseminated after synchronized ovulation with the Short Synch protocol. A total of 138 primi- and multiparous dairy cows from 5 dairy farms in North Macedonia were included in the study. At day 65±3 postpartum, an ovarian ultrasound examination was performed for presence of corpus luteum (CL), and body condition scoring (BCS) was made (scale from 1-emaciated to 5-obese). Cows with a CL (n=92) were submitted to two synchronization protocols and grouped accordingly: SPG (n=46) synchronized with a single PGF injection, and SSG (n=46) synchronized with a Short Sync protocol (PGF–24h–PGF–32h–GnRH–16h–TAI). Cows were followed up to the second insemination. Pregnancy diagnosis was done at d30 and d60 after each insemination. Eleven cows (n=11) from the SPG didn’t show estrus signs and were excluded from further analysis. Overall, a higher FR (p=0.0008) was observed in the SSG (71.74%, 33/46) compared to the SPG (34.29%, 12/35). Cows in the SSG with BCS>2.75 (76.19%, 16/21) had a higher FR (p=0.0016) than cows in the SPG with BCS>2.75. No differences were observed (p=0.1242) between both groups that had BCS≤2.75 (68.00%, 17/25; 43.75%, 7/16, respectively). Multiparous cows in the SSG had a higher FR (p=0.0021, 71.43%, 25/35) than their counterparts from the SPG (30.43%, 7/23). No significant differences were observed among the primiparous cows. The results indicate that Short Synch protocol might increase the fertility rate in dairy farms.

Keywords: BCS, cows, fertility rate, Short Synch


References

1. Djordjević, M., Blagojević, J., Radinović, M., Cincović, M., Maletić, J., Kreszinger, M., Maletić, M. (2022). Efficiency of two protocols of resynchronization of estrus and ovulation in highproducing dairy cows at peak lactation. Acta Vet. 72(3): 362-374. https://doi.org/10.2478/acve-2022-0029 
2. Kohsari, H., Berenjian, K., Heydarian, S. (2024). Reproductive performance improvement following the implementation of a modified Double-Ovsynch protocol compared to G6G in lactating Holstein cows. Vet Arhiv. 94(5): 353-364. https://doi.org/10.24099/vet.arhiv.1564 
3. Stevenson, J.S., Phatak, A.P. (2010). Rates of luteolysis and pregnancy in dairy cows after treatment with cloprostenol or dinoprost. Theriogenology 73(8): 1127-1138. https://doi.org/10.1016/j.theriogenology.2010.01.014 PMid:20096923
4. Stevenson, J.S. (2016). Physiological predictors of ovulation and pregnancy risk in a fixed-time artificial insemination program. J Dairy Sci. 99(12): 10077-10092. https://doi.org/10.3168/jds.2016-11247 PMid:27720155
5.Pursley, J.R., Mee, M.O., Wiltbank, M.C. (1995). Synchronization of ovulation in dairy cows using PGF and GnRH. Theriogenology 44(7): 915-923. https://doi.org/10.1016/0093-691X(95)00279-H PMid:16727787
6. Navanukraw, C., Redmer, D.A., Reynolds, L.P., Kirsch, D.J., Grazul-Bilska, A.T., Fricke, P.M. (2004). A Modified presynchronization protocol improves fertility to timed artificial insemination in lactating dairy cows. J Dairy Sci. 87(5): 1551-1557. https://doi.org/10.3168/jds.S0022-0302(04)73307-X PMid:15291005
7. Vasconcelos, J.L.M., Silcox, R.W., Rosa, G.J.M., Pursley, J.R., Wiltbank, M.C. (1999). Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. J Diary Sci. 52(6): 1067-1078. https://doi.org/10.1016/S0093-691X(99)00195-8 PMid:10735113
8. Ferguson, J.D., Galligan, D.T., Thomsen, N. (1994). Principal descriptors of body condition score in Holstein cows. J Dairy Sci. 77(9): 2695-2703. https://doi.org/10.3168/jds.S0022-0302(94)77212-X PMid:7814740
9. Atanasov, B., Hostens, M., Celeska, I., Ilieska, K., Opsomer, G., Dovenski, T. (2015). Follicular dynamics following induced luteolysis and transvaginal ultrasound-guided aspiration of the largest follicle in dairy cows. Vet Arhiv. 85(3): 247-260.
10. Wiltbank, M.C., Baez, G.M., Cochrane, F., Barletta, R.V., Trayford, C.R., Joseph, R.T. (2015). Effect of a second treatment with prostaglandin F2α during the Ovsynch protocol on luteolysis and pregnancy in dairy cows. J Dairy Sci. 98(12): 8644-8654. https://doi.org/10.3168/jds.2015-9353 PMid:26433418
11. Brusveen, D.J., Souza, A.H., Wiltbank, M.C. (2009). Effects of additional prostaglandin P2α and estradiol - 17β during Ovsynch in lactating dairy cows. J Dairy Sci. 92(4): 1412-1422. https://doi.org/10.3168/jds.2008-1289 PMid:19307622
12. Carvalho, P.D., Fuenzalida, M.J., Ricci, A., Souza, A.H., Barletta, R.V., Wiltbank, M.C., Fricke, P.M. (2015). Modification to Ovsynch improve fertility during resynchronization: Evaluation of resynchronization with gonadotropin-releasing hormone 6 d before initiation of Ovsynch and addition of a second prostaglandin P2α treatment. J Dairy Sci. 98(12): 8741-8752. https://doi.org/10.3168/jds.2015-9719 PMid:26433420
13. Sauls-Hiesterman, J.A., Voelz, B.E., Stevenson, J.S. (2020). A shortened resynchronization treatment for dairy cows after a nonpregnancy diagnosis. Theriogenology 141, 105-112. https://doi.org/10.1016/j.theriogenology.2019.09.013 PMid:31536859


Copyright

© 2025 Murdzeva E. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Conflict of Interest Statement

The authors declared that they have no potential lict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 48, Issue 1, Pages i-vii, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2025-0013