Original Scientific Article
Improved determination of macroscopic parasite preparations using s10 modified plastination procedure
Zoran Kocevski*,
Jovana Stefanovska,
Vlatko Ilieski,
Lazo Pendovski,
Elena Atanaskova

Mac Vet Rev 2010; 33 (2): 7 - 14

636.09.616.99

Received: 15 October 2010

Received in revised form: 15 October 2010

Accepted: 15 October 2010

Available Online First: 15 October 2010

Published on: 15 October 2010

Correspondence: Zoran Kocevski,

Abstract

Macroscopic preparations of parasites fixed in formaldehyde or alcohol don’t fulfill in complete the requests for education, as well as their determination, mainly because of the toxic fumes and not enough visible structure of fixed parasite. Using the modified С10 plastination method, parasites from three different phylum were prepared: Plathelminthes: Class Cestoda (Dipilidum caninum, Moniezia spp and larvae from T.Echinococcus granulosus - Echinococcus unilocularis, larvae from T. pisiformis - Cysticercus pisiformis, , larvae from T. hidatigena - Cysticercus tenuicollis), Phylum Nemathelminthes, Class Nematoda, (Ascaris suum, Macracanthorhynchus hirudinaceus, Diro filaria immitis), Phylum Arthropoda, Class Arachnida (tick from the Ixodidae family) and Class Insecta (Gasterophilus intestinalis, Hypoderma bovis). The aim of this study was conserving the parasites in native condition with plastination method and improved determination according to their visible morphologic structure. Parasites were previously kept in 10% formaldehyde. Prepared parasites were dry, chemical free, not toxic and safe for the environment, flexible and with detained form and structure. There was a variation in the natural colors in some of the parasites, as a result from long-time formalin fixation. Preparations made with this method are permanent educative material which enables improved study of parasite’s structure.

Keywords: Plastination; Parasites; Macroscopic preparation ; Determination


References


Citation Information

Macedonian Veterinary Review. Volume 33, Issue 2, Pages 7-14, p-ISSN 1409-7621, e-ISSN 1857-7415, 2010