Original Scientific Article
The effect of diet supplemented with Dandelion’s (Taraxacum officinale) extract on the productive and blood parameters of common carp (Cyprinus carpio I.) cultivated in the recirculation system
Ivaylo Sirakov*,
Katya Velichkova,
Stefka Stoyanova,
Georgi Zhelyazkov,
Yordan Staykov

Mac Vet Rev 2019; 42 (2): 131 - 139

10.2478/macvetrev-2019-0017

Received: 28 October 2018

Received in revised form: 09 April 2019

Accepted: 14 May 2019

Available Online First: 09 June 2019

Published on: 15 October 2019

Correspondence: Ivaylo Sirakov, ivailo_sir@abv.bg
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Abstract

The aim of the current study was to test the effect of a diet supplemented with dandelion’s (Taraxacum officinale) extract on the productive traits and blood parameters in common carp (Cyprinus carpio L.) cultivated in an recirculation aquaculture system. The carps were cultivated at a stocking density of 7.2 kg/m3 in recirculation aquaculture system. The fish were split into the following two experimental groups: DF (the fish were fed with feed supplemented with dandelion’s extract) and CF (the fish were fed with feed without supplementation). Common carp fed with a diet added with dandelion’s extract at a quantity of 0.8% from daily feed ratio, did not affect the hydrochemical parameters (pH, dissolved oxygen, and electrical conductivity). The carps fed with feed supplemented with dandelion’s extract did not show better productive traits compared with these found for carp from the control variant. The carp from experimental groups had a higher survival rate, final weight, average individual weight gain and specific growth rate (SGR), respectively with 13.2%, 3.94%, 31.5% and 31.3%, compared with the average values of these parameters measured in individuals fed with the control feed, but the differences were not statistically significant (P≥0.05). Supplementation of feed with dandelion’s extract significantly decreased the plasma cholesterol (4.76%) and triglyceride (61.2%) content, promoting hypolipidemic status in fish (P≤0.05).

Keywords: Dandelion’s extract, common carp, growth, blood parameters, RAS


INTRODUCTION

The recirculation aquaculture system (RAS) is a sustainable technology which is able to decrease the impact of aquaculture on the environment and to ensure environmentally friendly aqua production. These systems possess different advantages: they reduce water usage, improve waste management and nutrient recycling (1). Together with the advantages, however one of the most important disadvantages is the negative effect of high stocking density on fish health and growth (2, 3). Antibiotic substances could promote growth and improve fish health, thus solving in this way the problems described in RAS.
Antibiotics have been in wide use in fish farms ever since (4). Unfortunately, it was found that frequent use of antibiotics led to resistance in pathogen microorganisms, suppression of aquatic animal‘s immune system and pollution of the environment (5). One sustainable solution of the mentioned problems in RAS is the usage of extracts from different plants. It was reported that they could be used as an anti-stress, growth promoting, appetite-stimulating substances. It was found that they have hepatoprotective, immunostimulating effect and anti-pathogen properties in fish (6). The attractiveness of phytoextracts relies on their local availability and low price. Different plant extracts were tested for their growth promoting effect in aquaculture - Urtica dioica (7), Viscum album (7), Cynodon dactylon (8), Allium sativum (9), Satureja khuzestanica (10), Sauropus androgynus (11), Stacys lavandulifolia (12, 13). It was found that some of them together with providing improved appetite, digestibility, and availability of nutrients also showed an immunostimulating effect in cultivated fish (6).
The dandelion’s extract has been used in Bulgarian traditional medicine for hundreds of years as an appetite promoting substance and herbal medication for improving the condition of the gut, liver, and spleen. It contains various phytochemicals, including oligosaccharides, phenolic acids, polysaccharides, flavonoids, peptides, terpenes, and alkaloids. Most of them result in antibacterial, antioxidant, immune function modulating activities and anti-inflammatory activities in tested organisms (14). The effect of dietary supplementation of dandelion extract on growth and blood parameters was tested in chickens (15, 16), pigs (17, 18), rabbits (19). The investigations of its influence on growth and hematological parameters in fish are strongly limited (14, 20).
The aim of the current study was to test the effect of a diet supplemented with dandelion’s (Taraxacum officinale) extract on the productive traits and blood parameters in common carp (Cyprinus carpio L.) cultivated in a recirculation aquaculture system.


MATERIAL AND METHODS

Fish

Healthy common carps without visible injuries were chosen and transported from Fish farm Tundja 73, situated near at Nikolaevo town, to the Experimental aquaculture base situated at the Trakia University, Stara Zagora. The fish were split into the following experimental groups:
• Control groups, CF - carps were fed with feed without supplementation
• Experimental groups, DF – carps were fed with feed supplemented with dandelion’s extract

The average initial weight of fish from the two groups was:
• Experimental group, DF – 915.06 ± 139.8g
• Control group, CF – 908.5±171.2g

The experiment was carried out in two replications (16 specimens). The stocking density of fish was 7.2 kg/m3. The continuation of the trial was 45 days.
Animal management was approved by the ethics committee of Trakia University (acceptance of projects 3AF/18 and 8AF/18 ).


Figure 1. Recirculation aquaculture system: 1- fish tanks;. 2- mechanical filters; 3- moving bed biofilter; 4- heating elements; 5- pump tank; 6- pumps; 7- inlet water; 8- outlet water

Recirculation aquaculture system (RAS)

The recirculation system consists of fish tanks, two settling tanks as mechanical filters, moving bed biofilter, water heating and pump sections (Fig. 1). The useful volume of each fish tank was 0.8 m3. The flow rate in RAS was maintained at 133 l/min. The bottoms of fish tanks, as well as the bottoms of filter’s sections, were cleaned by siphoning every day. The volume of fresh water which was added daily for compensation of losses from the cleaning process and from evaporation was 20% of the total volume of RAS. The heating elements were not used during the current trial because the water temperature was optimal for the raised carps.

Fish feed

The experimental carps were fed with a commercial pelleted feed, produced by „Top mix“ with the size of pellets 6 mm. Its content could be seen in Table 1. The dandelion’s extract (produced by Nature’s way) was added to the pellets at a concentration of 0.8% of the daily feed rate. The sunflower oil at a quantity of 5 ml was also added to every 100 g of experimental feed and the components were very well mixed. The same amount of sunflower oil was added to the control feed (to ensure the same feed energy) and the pellets and oil were mixed. Afterward, both tested feeds were left for 12 hours at an air temperature of 20°C and were used for the feeding of carps. The daily feed rate was maintained at 1.8% of the total fish biomass. The feed was supplied manually three times a day (8.00 am, 12.00 am, 16.30 pm).

Table 1. Nutritional content of commercial feedused in the trial based on “Top mix” manufacture



Investigated parameters: Hydrochemical analysis

The temperature (°C), pH, dissolved oxygen (mg/l) and electrical conductivity (μS/cm) were measured daily with a portable meter HQ30D (Hach Lange, Germany country and company name, both), connected with a probe appropriate for each parameter. The difference in the value of average water’s temperature during the trial between experimental and control tanks was 0.1°C and it was not statistically proven (P≥0.05) (Table. 2). The measured average value of oxygen in tanks from the DF variant was 6.62 mg/l (Table. 2) and it was 6.3% higher than the average value measured for the control group CF, but the difference was not statistically significant (P≥0.05). The differences between the average values of pH and conductivity between both tested variants DF and CF were minimal and not statistically proven (Table. 2) (P≥0.05).

Table 2. Average value of hydrochemical parameters during the trial



Technological parameters

The mortality cases in experimental tanks were recorded during the trial and survival (%) was calculated using the following formulae:

image
The experimental fish were individually weighed at the technical balance with accuracy of 0,01g at the start and the end of the trial. On this basis, the average individual weight gain (WG) (g) and specific growth rate (SGR ) (% / day) were determined by the following equations:

WG =average final weight – average initial weight

image

At the end of trial feed conversion ratio (FCR) was also calculated by the following equation:

image

Measurement of blood parameters

The blood was taken directly from the hearts of the examined fish (12 specimens from the experimental and the control group) with disposable sterile plastic syringes (3 ml) with a needle. Heparin sodium (1%) was used as an anticoagulant. Half of the blood was centrifuged at 1006 g for separating the plasma for determination of biochemical parameters and the remaining blood was used for hematological analysis. Glucose (GLU) (mmol/l), urea (UREA) (mmol/l), creatinine (CREA) (μmol/l), total protein (TP) (g/l), albumin (ALB) (g/l), alanine aminotransferase (ALAT) (U/l), aspartate aminotransferase (ASAT) U/l), the content of calcium (Ca) (mmol/l), phosphorus (P) (mmol/l), magnesium (Mg) (mmol/l), as well as triglyceride (TG) (mmol/l) and cholesterol (mmol/l) in blood plasma, were examined by the colorimetric method with blood analyzer (Mindray SC – 120), (21). The numbers of white blood cells (WBC) (n/l), red blood cells (RBC) (n/l) were determined according to Ranzani-Paiva et al. (22). The content of hemoglobin (Hb) (g/l) was measured spectrophotometrically according to Hayatbakhsh et al. (23). The hematocrit value (%) was determined according to Ejraei et al. (24).

The blood parameters MCV, MCH and MCHC were calculated with the following equations:

image

Statistical analysis of data

The data were analyzed statistically with two methods: ANOVA single factor and independent T-test STATISTICA 6.0 software (StatSoft Inc., 2002) and SPSS version 17 (IBM Inc.).

image

Figure 2. Average values of productive parameters in common carp during the trial: A) survival; B) initial weight; final weight; D) average individual weight gain; E) SGR; F) FCR


RESULTS

Technological parameters

The survival rate of carps from the experimental variant DF was by 13.2% higher than the rate for fish from the CF (Fig. 2), without being statistically proven (P≥0.05).
The statistical differences in fish weights at the beginning of the trial from different experimental variants were not significant (P≥0.05). At the end of the experiment, the carp fed with feed supplemented with dandelion’s extract showed higher final weight, average individual weight gain and specific growth rate (SGR) respectively with 3.94%, 31.5% and 31.3% compared with the average values in these parameters found out in carps fed with control feed, but the differences were not statistically significant (P≥ 0.05).
Better FCR was found in carps from the DF variant and the average value of this parameter was lower by 9.7% compared with the average value of FCR found for fish fed with feed without supplementation (Fig. 2). Unfortunately, the difference was not statistically significant (P≥0.05)
Blood parameters

Attheendofthetrial, thebiochemicalparameters of blood (urea, creatinine, albumin, total protein, Ca, Mg) in carp from the experimental group DF showed higher values for these parameters compared with the ones in fish from the CF variant, but the differences were not statistically proven (P≤0.05) (Table 3). Lower average values in glucose, ALAT, phosphorus, triglyceride and cholesterol were found for the carps fed with supplemented feed, compared with the ones of individuals from the control group (CF), while the differences were statistically significant for triglyceride and cholesterol content (P≤0.05) (Table 3). The values of these parameters in fish plasma from the experimental group (fed with dandelion’s extract) were lower respectively by 61.2% and 4.76% compared with their values found for carps from the control variant.
Table 3. Average value of hematological and biochemical parameters in carp’s blood at the end of trial
image

The hematological parameters (RBC, hemoglobin and hematocrit) showed higher values in the blood of common carp from the CF variant, compared withtheones offishfrom theexperimental group, but statistically significant was only the difference found with regards to the hematocrit (P≤0.05). The hematocrit in carps from the DF was higher by 23.4% compared with the quantity found in the blood of individuals from the CF. The number of white blood cell (WBC) in carps fed with feed supplemented with the extract from dandelion were 17.5% lower compared with the values in this parameter for fish from the control variant, but the difference was not statistically significant (Table 3) (P≥0.05). The highеst Mean Corpuscular Volume (MCV) was observed in group DF (177.2±5.67 fL), while the lowest MCV value (152.6±6.06 fL) was observed in group CF. Analysis of the variance of MCV values showed a significant difference (P <0.05). The mean corpuscular haemoglobin (MCH) in carp from both experimental groups were without statistical differences (P≥0.05) (Table 3). The highest haemoglobin concentration (MCHC) was observed in group CF (1000.88±312.98 g/dl), while the lowest MCHC was observed in the group DF (777.72±216.69 g/dl). The statistical analysis of the MCHC values from both groups showed a significant difference (P <0.05).

DISCUSSION

Technological parameters

The plant’s extracts have shown to be promoting growth, stimulating appetite, reducing stress, as well as possessing anti-microbial properties in fish. Supplements are highly recommended for the purposes of aquaculture and their administration in fish farms has become routine practice (6). The survival rate in carps, fed with dandelion’s extract supplemented diet, was not affected by dandelion supplementation in the current trial compared with the rate found for the fish from the control variant, which could be a result of antibacterial and higher immune activity of the used additive. Different in vitro studies showed that the extract from dandelion possesses antimicrobial activity against microorganisms from the genera Escherichia, Bacillus and Staphylococcus (25, 26). Challenging tests in juvenile golden pompano (Trachinotus ovatus) with Vibrio harveyi, fed with diet supplemented with dandelion’s extract, showed better survival compared to fish from the control group.
The supplementation of fish and shrimps’ feed with plant extracts, increase their growth (6). In the current trial, the values of growth parameters (final weight, average individual weight gain and SGR) and feed conversion ratio (FCR) in fish from the DF group were higher than those found for the carps from the control group, but the differences were not statistically significant (P≥0.05). These results are in confirmation of results, received from Yun et al. (18), who found that feed for juvenile rockfish (Sebastes schlegelii), supplemented with dandelion additive did not affect their growth and feed efficiency (FE). Converse results were found by Tan et al. (12) in juvenile golden pompano (Trachinotus ovatus), where supplementation with dandelion’s extract enhanced the growth performance, feed intake and FE. The authors stated that the dosage of dandelion’s extract over 1.0 g/kg could result in a toxic effect and stress in fish that leads to a decrease in growth and feed utilization. The possible explanation of the differences in results received from Tan et al. (12) and the current study could be that the common carp is reaching this limit dose at a lower level than the one found for the golden pompano (Trachinotus auratus). Future studies are necessary for the optimal dose of dandelion’s additive for carp’s feeding to be determined.
Blood parameters

The blood parameters in carps were affected at a higher degree from the substitution of dandelion’s extract, than the growth parameters. The addition of different supplements to the fish diet could strongly affect these parameters, which could be used as an indicator of health status in fish (27). The lower quantity of glucose (50.3%) in carps, fed with feed supplemented with dandelion, compared to the value of this parameter in fish from the CF was not proved statistically. The dandelion extracts possess inhibitory activity on a-glucosidase activity, as well as on the antihyperglycemic activity of diabetic mice (28).
A higher level of plasma TP (21.6%) is found in fish, fed with feed supplemented with dandelion’s extract, compared with the one found in carp fed with feed without supplementation. Unfortunately, this difference was not statistically significant (P≥0.05). Tan et al. (12) found a significantly higher level of plasma TP and alkaline phosphatase in golden pompano when it was fed with the addition of dandelion’s extract and associate their high level with stronger innate immunity response. The significantly lower level of TG and CHO (P≤0.05) in blood plasma in the current study is in confirmation of results received from Tan et al. (12) in golden pompano fed with dandelion supplemented feed, which stated that dandelion’s addition in feed possesses a hypolipidemic capacity in fish. Similar results connected with the reduction of triglycerides and cholesterol in the blood of common carp were found when wood betony (Stachys lavandulifolia) were administered in fish feed (13).
White blood cells assure protection of organisms against pathogens and toxic compounds. Increased number of WBC in fish showed an inflammatory process (29). The received results for the number of white blood cells in the current study were contrary to results received from Abasali and Mohamad (30), where different herbal immune stimulant diets were used and which led to enhanced WBC’s levels. The number of red blood cells, hemoglobin and hematocrit indicate the oxygen-carrying capacity of fish (31). The RBC, hemoglobin were not affected by the supplementation of dandelion, but hematocrit in carps from the CF variant was higher compared with its quantity in fish from the experimental group, which could result from the lower quantity of oxygen measured in control tanks during the experiment. The Mean Corpuscular Volume (MCV) and the mean haemoglobin concentration (MCHC) in fish blood were affected from supplementation of carp feed with dandelion’s extract. The effect of Taraxacum officinale on blood parameters MCH, MCV, MCHC was not retraced in other studies. The effect of supplementation of different medicinal plants (Aloe vera, Origanum vulgare) in fish feed on these parameters in rainbow trout did not show differences compared with the values in MCH, MCV and MCHC found for the fish from the control group (32, 33).
The main mechanism of action in dandelion’s supplementation in feed is connected with stimulation of the digestive system and its diuretic effect. It was reported that dandelion showcases several beneficial effects on the intestinal mucosa in poultry regarding the architecture of the villi, villus height/ crypt depth ratio, as well as cellular infiltration. Unfortunately, this simulative effect was not found in the current study on common carp and only blood parameters were affected from the dandelion supplementation (34).

CONCLUSION

In conclusion, the current study found that common carp (Cyprinus carpio) fed with a diet supplemented with dandelion extracts at 0.8% did not affect hydrochemical parameters (pH, dissolved oxygen and conductivity). The carps fed with added dandelion’s extract showed higher survival rate, final weight, average individual weight gain and specific growth rate (SGR), respectively with 13.2%, 3.94%, 31.5% and 31.3%, compared with the average values in these parameters found in fish fed with control feed. The differences in survival and growth parameters of the two tested variants were not statistically significant (P≥0.05). Supplementation of feed with dandelion’s extract significantly decreased plasma cholesterol and triglyceride content, thus promoting a hypolipidemic status in fish (P≤0.05).

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Copyright

© 2019 Sirakov I. This is an open-access article publishedunder the terms of the Creative Commons Attribution License whichpermits unrestricted use, distribution, and reproduction in any medium,provided the original author and source are credited.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 42, Issue 2, Pages 131-139, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2019-0017, 2019