The aim of the study was to determine the presence and distribution or to confirm the absence of avian influenca viruses in farmed poultry in the poultry production systems 1 and 2 from the eight statistical regions in R. Macedonia. Total number of 1215 cloacal swabs from poultry were sampled. Each sample was processed and analysed by both molecular (RRT-PCR) and classical virology methods (virus isolation and identification). All samples gave negative result for presence of avian influenza viruses. Commercial poultry production systems have biosecurity measures preventing the entry of pathogens i.e avian influenza viruses, therefore resulting with no circulation of these viruses in the sampled farmed poultry flocks.