Original Scientific Article
Investigation of some hematological and blood biochemical parameters in cattle spontaneously infected with bovine leukosis virus
Sandev Nikolay * ,
Zapryanova Dimitrinka ,
Stoycheva Ivanka ,
Rusenova Nikolina ,
Mircheva Teodora

Mac Vet Rev 2013; 36 (2): 107 - 110

636.2.09.[616.155.392-022.578.828.11

Received: 28 June 2013

Received in revised form: 30 July 3013

Accepted: 26 August 2013

Available Online First: 15 October 2013

Published on: 15 October 2013

Correspondence: Sandev Nikolay, n_sandev@abv.bg
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Abstract

The aim of the present study was to follow out the alterations in some haematological and blood biochemical parameters in cattle spontaneously infected with enzootic bovine leukosis virus with regard to the invivodifferentiation of bovine leukosis stages. The experiment included 76 cows at various ages and body weight. Serological leukosis tests were done by agar-gel immunodiffusion test with a commercial kit of Synbiotiсs (France), containing standardised gp 51 antigen and positive serum approved by the EU. On the basis of haematological results, the cows were divided into three groups: first group – EBL-seropositive with normal haemogramme; second group – EBL seropositive with altered haemogramme and third group – controls. In cows from the first and the second group, a statistically significantly increased blood cell counts was established compared to healthy controls. The total WBC were increased in the second group (leukocytosis) up to 33.21×109/l vs reference range of 5-10×109/l as well as lymphocyte percentages (lymphocytosis) – 81.89% (reference 40–63%). A reduction in the proportion of neutrophils to 12.78% (relative neutropenia) vs the reference range of 22-49% and monocytes (monocytopenia) to 1.78% (reference range 2–6%) was observed. A statistically significant reduction in Ca concentrations (4.41 mg/dl) and higher inorganic phosphate levels (5.28 mg/dl) were established in cows from the second group. Also, ASAT activity was considerably lower – 47.03 U/l, while alkaline phosphatase increased slightly within the reference range up to 167.68 U/l and 165.81 U/l in groups one and two, respectively. The present haematological and whole blood/serum biochemical results in cows spontaneously infected with EBL virus could be used as prognostic markers of the course of the disease, to distinguish the stages of infection with regard to alive diagnostics.

Keywords: enzootic bovine leukosis, cows, haematological and blood biochemical assays


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Copyright

©2013 Sandev N. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 36, Issue 2, Pages 107-110, p-ISSN 1409-7621, e-ISSN 1857-7415, 2013