Original Scientific Article
Biliary clearance of bromosulfophthalein in healthy and ketotic Holstein cows
Kirovski Danijela * ,
Sladojević Željko ,
Šamanc Horea

Mac Vet Rev 2013; 36 (2): 86 - 89

636.2.09.616-008.9

Received: 30 June 2013

Received in revised form: 14 July 2013

Accepted: 20 August 2013

Available Online First: 15 October 2013

Published on: 15 October 2013

Correspondence: Kirovski Danijela, dani@vet.bg.ac.rs
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Abstract

Ketosis is a metabolic disorder closely associated with liver lipidosis. Numerous tests have been developed to detect hepatic dysfunction in dairy cows. Bromosulfophthalein (BSP) clearance is established as a sensitive index of hepatic function. The objective of this study was to examine the difference of biliary excretion of BSP between ketotic and healthy Holstein cows and to correlate this excretion with other indicators of liver dysfunction. Twenty puerperal Holstein cows divided in two groups (10 cows each) were involved in the study. The first group included healthy and the second group ketotic cows. Blood samples were taken 10 days after parturition. Concentrations of total protein, albumin, total bilirubin, Ca, P, total lipids, urea and glucose were determined. Immediately after blood sampling, BSP test was performed. Blood samples were taken 5 and 45 minutes after injection, and the percentage of retained pigment in the sample obtained at minute 45 was calculated. Blood albumin and glucose concentrations were significantly higher in healthy then ketotic cows. Total bilirubin concentration was significantly higher in ketotic than healthy cows. BSP excretion was significantly higher in ketotic compared to healthy cows. There was a significant positive correlation between BSP values and total bilirubin concentrartions in both healthy and ketotic cows and a significant negative correlation between BSP values and glucose concentrartions in both healthy and ketotic cows.  In conclusion, biliary clearance of BSP may be used as a reliable method for the detection of hepatic dysfunction associated with clinical symptoms of ketosis in dairy cows.

Keywords: Holstein cows, BSP test, ketosis


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Copyright

©2013 Kirovski D. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 36, Issue 2, Pages 86-89, p-ISSN 1409-7621, e-ISSN 1857-7415, 2013