Original Scientific Article
Study of the effects of breed on some innate immunity parameters in rams
Genova Krasimira * ,
Dimitrova Ivona ,
Stancheva Nevyana ,
Angelov Geno ,
Nakev Jivko ,
Mehmedov Tandju ,
Georgieva Svetlana

Mac Vet Rev 2013; 36 (2): 111 - 115

636.32/.38.09.612.017.1

Received: 06 June 2013

Received in revised form: 23 July 2013

Accepted: 27 August 2013

Available Online First: 15 October 2013

Published on: 15 October 2013

Correspondence: Genova Krasimira, dr_kig@abv.bg
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Abstract

Investigations were carried out on 26 rams from the breeds Karakachan and Copper-Red  Shoumen. The non-specific immune parameters,  phagocytic activity of leukocytes, bactericidal activity of phagocytes systems (oxygen-dependent and oxygen independent) and total plasma protein level were evaluated. Phagocytic response was evaluated against S. aureus 209-P with a certain percentage of active phagocytes (phagocytic index) and the number of absorbed particles per one phagocytic cells (phagocyte number).  Phagocytosis completion index was defined as the percentage of the microbial cells that have been destroyed by phagocytes after incubation. State of the oxygen-dependent bactericidal systems of phagocytes was assessed in vitro using the NBT test, which reflects the ability of superoxide restore NBT in diphormazane. NBT test was evaluated by the degree of reduction in spontaneous and stimulated reactions, taking into account the intracellular deposits diphormazane. Our studies and results shows that the rams from the two local Bulgarian breeds have a high activity of innate immune parameters and that’s may be useful and important in the breeding programs as an indicator of resistance and highly tolerance to oxidative stress.

Keywords: sheep, indigenous breeds, phagocytic activity, NBT test, total plasma protein


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Copyright

©2013 Genova K. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Acknowledgement

Research was part of the project 501/22- 05.12.2012 (2012-2014) “Development of DNA markers (CAST, MSTN) for fattening ability and meat quality in Synthetic population Bulgarian Milk, Karakachan and Copper Red Shumen sheep breeds” financed by the Ministry of Education, Youth and Science, Republic of Bulgaria.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 36, Issue 2, Pages 111-115, p-ISSN 1409-7621, e-ISSN 1857-7415, 2013