Original Scientific Article
Validation of screening method for determination of methyltestosterone in fish
Uzunov Risto
*
,
Hajrulai-Musliu Zehra
,
Stojanovska-Dimzoska Biljana
,
Dimitrieska-Stojkovic Elizabeta
,
Todorovic Aleksandra
,
Stojkovski Velimir
Received: 09 March 2013
Received in revised form: 28 June 2013
Accepted: 05 July 2013
Available Online First: 15 March 2013
Published on: 15 March 2013
Correspondence: Uzunov Risto, risteuzunov@gmail.com
Abstract
Anabolic androgenic steroids are synthetic derivatives of testosterone, which is the primary male sex hormone. These anabolic agents are used to increase the weight gain, to improve the food efficiency, storing proteins and to decrease fatness. However, depending on the use of anabolic agent in animal feed, anabolic residues that may occur in meat and meat products present risks to human health. The aim of this study was the validation of screening ELISA method for determination of methyltestoterone anabolic steroid in fish. The validation process was carried out according to Commission Decision 2002/657/EC criteria. The detection limit for methyltestosterone was 140.95 ng/kg and the detection capability was 564.43 ng/kg. The overall recoveries and the coefficients of variation (CV) were in the range of 82.4%-97.4% and 1.5%-6.9%, respectively, a working range between 50 to 4050 ng/kg, and the regression equation of the final inhibition curve was: y= -0,1741x + 1,5082, R2 = 0.9927. Because of the good recovery and precision, and satisfactory detection capability, this method is applicable in official control laboratories as a rapid screening method for determination of methyltestosterone in fish.
Keywords: methyltestosterone, fish, ELISA, anabolic steroids, validation
Citation Information
Macedonian Veterinary Review. Volume 36, Issue 1, Pages 19-23, p-ISSN 1409-7621, e-ISSN 1857-7415, 2013
639.3.577.175.6.083.3
