An analytical method based on immunoaffinity column (IAC) clean-up and quantitative determination with liquid chromatography - fluorescence detection (LC-FD) was used to determine the occurrence of ochratoxin A (OTA) in Macedonian wines originated from the most famous, Tikve{, wine producing region. The linearity of the method was checked, and a good coefficient of correlation (0.9976) was found, over wide concentration range of 0,1 to 60 ng/ml. The limit of detection and quantification were 0,043 ng/ml and 0,131 ng/ml, respectively. The accuracy of the method was determined with spiked OTA free samples at the concentration levels of 0.1 ng/ml and 1.0 ng/ml. The recoveries for red wine were found to be 100,66% and 106,83 %, for the proposed spiking levels. The recoveries for white wine were satisfactory (98,50% and 93,31%) too, in the same spiking levels. RSD values for both, red and white wine samples, for the proposed concentration level was satisfactory, in the rang of 7,13% to 10,41% for red wine and 3,96% to 8,77% for white wine samples. A survey was done on 31 bottled wine samples. Among them, 16 samples (51,61%) were with OTA concentration over the LOD. However, the determined concentrations were too low to present a risk for wine consumers.