Original Scientific Article
Appllicability of a spectrophotometric method for assessment of oxidative stress in poultry
Petra Regenhard
*
,
Dimitar Nakov
,
Helga Sauerwein
Received: 22 July 2013
Received in revised form: 09 October 2013
Accepted: 16 October 2013
Available Online First: 15 November 2013
Published on: 15 March 2014
Correspondence: Petra Regenhard,
Abstract
Oxidative stress is discussed as being related to health, welfare, and also to product quality. Recently, a spectrophotometric method based on the Fenton reaction (reactive oxygen metabolites, ROM) has been proposed to characterize pro-oxidative stressors in body fluids by determining early products of oxidation from proteins, lipids and nucleic acids. The applicability of this method for blood samples from various bird species was tested. The validity of the method for mammalian domestic animals was demonstrated by linearity yielded with increasing amounts of serum. In contrast, serum obtained from chicken yielded ROM concentrations below the limit of detection when serum volumes comparable to mammalian samples were used. Applying higher amounts of serum was not increasing the optical density readings in a linear way. To assess whether the lack of sensitivity and linearity is a problem limited to chicken or poultry in general, we tested sera from laying hens, turkey, duck, goose, pheasant, pigeon, and guinea fowl. For most of the species tested, the method could not be validly applied. There is no reason to assume that oxidative stress in birds may not exist, therefore we conclude that bird specific matrix effects hinder the valid application of the ROMs test in poultry.
Keywords: oxidative stress, dROM test, poultry
References
1. Knight, J.A. (2000). Review: Free Radicals, Antioxidants, and the Immune System. Ann Clin Lab Sci; 30 (2): 145-158.
2. Abuja, P.M., Albertini, R. (2001). Methods for monitoring oxidative stress, lipid peroxidation and oxidation resistance of lipoproteins. Clin Chim Acta 306: 1-17.
3. Brambilla, G., Civitareale, C., Ballerini, A., et al. (2002). Response to oxidative stress as a welfare parameter in swine. Redox Rep 7: 159-163.
4. Botsoglou, N.A., Fletouris, D.J., Papageorgiou, G.E., Vassilopoulus, V.N., Mantis, A.J., Trakatellis, A.G. (1994). Rapid, sensitive and specific thiobarbituric acid method for measuring lipid peroxidation in animal tissue, food and feedstuff samples. J of Agric Food Chem 42: 1931-1937.
5. Jo, C., Ahn, D.U. (1998). Fluorometric analysis of 2-thiobarbituric acid reactive substances in turkey.Poult Sci 77(3): 475-480.
6. Young, J.F., Stagsted, J., Jensen, S.K., Karlsson, A.H., Henckel, P. (2003). Ascorbic acid, alpha-tocopherol, and oregano supplements reduce stress-induced deterioration of chicken meat quality . Poult Sci 82(8): 1343-1361.
7. Cesarone, M.R., Belcaro, G., Caratelli, M., et al. (1999). A simple test to monitor oxidative stress. Int Angiol 18(2): 127-130.
8. Alberti, A., Bolognini, L., Macciantelli, D. (2000). The radical cation of N,N-Dienthyl-paraphenylendiamine: a possible indicator of oxidation stress in biological samples. Research Chemical Intermedicine 26: 253-267.
9. Carlucci, F., Tabucchi, A., Biagioli, B., et al. (2002). Cardiac surgery: myocardial energy balance, antioxidant status and endothelial function after ischemia-reperfusion. Biomed Pharmacother 56: 483-491.
10. Campise, M., Bamonti, F., Novembrino, C., et al. (2003). Oxidative stress in kidney transplant patients. Transplantation 76: 1474-1478.
11. Christou, K., Markoulis, N., Moulas, A.N., Pastaka, C., Gourgoulianis, K.I. (2003). Reactive oxygen metabolites (ROMs) as an index of oxidative stress in obstructive sleep apnea patients. Sleep Breath 7: 105-110.
12. Brambilla, G., Fiori, M., Archetti, L.I. (2001). Evaluation of the oxidative stress in growing pigs by microplate assays. J Vet Med A Physiol Pathol Clin Med. 48(1): 33-38.
13. Ballerini, A., Civitareale, C., Fiori, M., Regini, M., Betti, M., Brambilla, G. (2003). Traceability of inbred and crossbred Cinta Senese pigs by evaluating the oxidative stress. J Vet Med A Physiol Pathol Clin Med 50: 113-116.
14. Shapiro, F., Mahagna, M., Nir, I. (1997). Stunting effect in broilers; effect of glucose or maltose supplementation on digestive organs, intestinal disaccharidases, and some blood metabolites. Poult Sci 1997; 75:369-380.
15. Simoyi, M., Van Dyke, K., Klandorf, H. (2002). Manipulation of plasma uric acid broiler chicks and its effect on leukocyte oxidative activity. Am J Physiol;282: 791-796.
16. Trotti, R., Carratelli, M., Barbieri, M., et al. (2001). Oxidative stress and a thrombophilic condition in alcoholics without severe liver disease. Haematologica 86: 85-91.
17. Rekitt, M., Sauerwein, H., Andresen, U. (2002). Orientierende Untersuchungen zum oxidativen Stress bei Reitpferden. Tierärztl. Umsch 57: 471-481.
18. Rekitt, M., Staub, M., Andresen, U., Sauerwein, H. (2003). Orientierende Untersuchungen zum oxidativen Stress bei Hunden. Tierärztl Umsch 58: 70-78.
19. Andresen, U., Rekitt, M., Sauerwein, H. (2003). Orientierende Untersuchungen zum oxidativen Stress bei Milchkühen und zur Wirksamkeit peripartaler intramuskulärer Gaben von Vitamin E/Selen. Tierärztl Umsch 58: 467-472.
20. Sauerwein, H., Schmitz, S., Hiss, S. (2005). The acute phase protein haptoglobin and its relation to oxidative status in piglets undergoing weaning-induced stress. Redox Report 10(6): 295-302.
21. Celi, P., Selle, P.H., Cowieson, A.J. (2013). The effects of dietary supplementation with different organic selenium sources on oxidative stress in broilers. 24th Aust. Poult. Sci. Symp. 17th – 20th February 2013, 76-79.
22. Palmieri, B., Sblendorio, V. (2007). Oxidative stress tests: overview on reliability and use, Part II. European Review for Medical and Pharmacological Sciences, 11:383-399
23. Beitz, D.C. (2004) Protein and Amino Acid Metabolism. In: Reece WO, ed. Dukes Physiology of Domestic Animals. Cornell University Press, Ithaca, USA, 535-552.
24. Stinefelt, B., Leonard, S.S., Blemings, K.P., Shi, X., Klandorf, H. (2005). Free radical scavenging, DNA protection, and inhibition of lipid peroxidation mediated by uric acid. Annals of Clinical and Laboratory Science, vol. 35, no. 1, 37-45.
25. Carro, M.D., Falkenstein, E., Radke, W.J., Klandorf, H. (2010). Effects of allopurinol on uric acid concentrations, xanthine oxidoreductase activity and oxidative stress in broiler chickens. Comparative Biochemistry and Physiology, Part C, 151: 12–17.
26. Klandorf H, Rathore D, Iqbal M, Shi X, Simoyi M, Van Dyke K. (2002). Acceleration of tissue aging in chickens caused by oxidative stress using allopurinol and detected by cellular humoral chemiluminescence. In: Luminescence Biotechnology (Van Dyke K, Van Dyke C, Woodfork K, Eds), CRC Press, New York,; pp 393-407.
27. Simic M, Jovanovic S. (1989). Antioxidation mechanisms of uric acid. J Amer Chem Soc; 111:5778-5782.
28. Santos C, Anjos E, Augusto O. (1999). Uric acid oxid-ation by peroxynitrite: multiple reactions, free radical formation, and amplification of lipid oxidation. Arch Biochem Biophys; 372:285-294.
Copyright
© 2014 Sauerwein H. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Acknowledgment
We thank Ms. Barbara Heitkönig for excellent technical assistance. The scholarship of the German Academic Exchange Service (DAAD) awarded to Mr. Dimitar Nakov is gratefully acknowledged.
Conflict of Interest Statement
The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.
Citation Information
Macedonian Veterinary Review. Volume 37, Issue 1, Pages 43-47, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.14432/j.macvetrev.2013.10.002, 2014
10.14432/j.macvetrev.2013.10.002
