Original Scientific Article
Improvement of the antioxidative status of pig ovaries by selenopyran treatment
Desislava Vasileva Abadjieva
*
,
Elena K. Kistanova
,
Yordan Marchev
,
Radka Nedeva
,
Chaika Vaisberg
,
Rossen Georgiev Stefanov
,
Gennadiy Boryaev
,
Mihail Nevitov
Received: 02 June 2014
Received in revised form: 31 July 2014
Accepted: 10 August 2014
Available Online First: 14 August 2014
Published on: 15 October 2014
Correspondence: Desislava Vasileva Abadjieva, dessi_l@abv.bg
Abstract
Selenium is an essential micro-element in animal diet due to its high antioxidative properties. As a part of selenocystein it is an important constituent of the glutathione peroxidase (GPx) enzyme, which has a big importance for cell protection from oxidative damage. The aim of the present work was the investigation of the selenopyran effect on the antioxidative state of the pig ovary. The experiment was conducted with 18 gilts of Danube white breed randomly divided into two groups between 120 - 228 days of age. The animals received equal basal diets without selenium additives. The experimental gilts were injected once per month intramuscularly with oil solution of preparation selenopyran (9-phenylsymmetrical octahydroselenoxanthene) ensured 0.1 mgSe/kg live weight. After slaughtering, the ovaries were used for histological analysis and estimation of the selenium content in ovarian tissue by the atomic absorption spectroscopy method. The GPx activity in ovary homogenates using the colorimetric assay kit (BioVision) was measured. The expression of γ-glutamyl transpeptidase (GGT) in ovaries by immunochistochemical method was estimated. The selenopyran treatment leads to signifi cant (P<0.05) increase of the selenium level in blood and non-signifi cant (P>0.05) in ovarian tissue. Enhancement of GPx activity in the ovaries of experimental group was observed (142.61±6.6 versus 122.28±3.4U/gP, P<0.05). The GGT expression in the ovarian cortex cells, follicular fl uid and in the erythrocytes of ovarian blood vessels in treated gilts was an evidence of active transport of glutathione from blood to the ovary tissue. The selenopyran treatment promotes the increase of the GPx dependent antioxidative defense in ovary of growing gilts.
Keywords: pig ovary, selenopyran, glutathione peroxidase (GPx)
References
1. Reilly, C. (2006). Selenium in food and health. New York: Springer.
2. Drasch, G., Mail, Der. S., Schlosser, C., Roider, G. (2000). Content of non-mercury-associated selenium in human tissues. Biol Trace Elem Res., 77, 219-230.
http://dx.doi.org/10.1385/BTER:77:3:219
3. Kohrle, J., Jakob, F., Contempre, B., Dumont, J.E. (2005). Selenium, the thyroid, and the endocrine system. Endocr Rev., 26, 944-984. http://dx.doi.org/10.1210/er.2001-0034 PMid:16174820
4. Gladyshev, Vadim N. (2006). Selenoproteins and selenoproteomes. Vadim Gladyshev Publications. Paper 36, p.102.
5. Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G., Hoekstra, W.G. (1973). Selenium: biochemical role as a component of glutathione peroxidase. Science, 179, 588-590. http://dx.doi.org/10.1126/science.179.4073.588 PMid:4686466
8. Ozkaya, M.O., Naziroglu, M., Barak, C., Berkkanoglu, M. (2010). Effects of multivitamin/mineral supplementation on trace element levels in serum and follicular fluid of women undergoing in vitro fertilization (IVF). Biol. Trace Elem. Res., 139, p. 1-9. http://dx.doi.org/10.1007/s12011-010-8637-x PMid:20180042
9. Segerson, E. C., F. A., Murray, A. L., Moxon, D., Redman, R., Conrad, H. R. (1977). Selenium/vitamin E: Role in fertilization of bovine ova. J. Dairy Sci., 60, 1001–1005.
http://dx.doi.org/10.3168/jds.S0022-0302(77)83978-7
10. Segerson, E. C., Ganapathy, S. N. (1980). Fertilization of ova in selenium/vitamin E-treated ewes maintained on two planes of nutrition. J. Anim. Sci., 51, 386–394. PMid:7440436
11. Mahan, D. C. (2000). Effect of organic and inorganic selenium sources and levels on sow colostrums and milk selenium content. J. Anim. Sci., 78,100-105. PMid:10682808
12. Mahan, D. C., Peters, J. C. (2004). Long-term effects of dietary organic and inorganic selenium sources and levels on reproducing sows and their progeny. J. Anim. Sci., 82,1343-1358. PMid:15144075
13. Yoon, I., McMillan, E. (2006). Comparative effects of organic and inorganic selenium on selenium transfer from sows to nursing pigs. J. Anim. Sci., 84, 1729-1733.
http://dx.doi.org/10.2527/jas.2005-311 PMid:16775056
15. Ibrishimov, N., Lalov, Hr. (1984). Clinical laboratory research in veterinary medicine. Zemizdat, Sofia.
16. Niida, S., Kawahara, M., Ishizuka, Y., Ikeda, Y., Kondo, T., Hibi, T., Suzuki, Y., Ikeda, K., Taniguchi, N. (2004). Gamma-glutamyl transpeptidase stimulates receptor activator of nuclear factor kappa B ligand expression independent of its enzymatic activity and serves as a pathological bone-resorbing factor. J. Biol. Chem., 279, 5752–5756.
http://dx.doi.org/10.1074/jbc.M311905200 PMid:14634009
17. Flohe, L. (1997). Selenium in peroxide metabolism. Med. Klin., 92, 5–7.
19. Giguere, A., Fortier, M.-E., Matte, J. J. (2005). Rapid, sensitive and versatile determination of selenium in different biological samples. Can. J. Anim. Sci., 85, 533–536.
http://dx.doi.org/10.4141/A05-044
20. Payne, R. L., Southern, L. L. (2005). Comparison of inorganic and organic selenium sources for broilers. Poult. Sci., 84, 898–902. http://dx.doi.org/10.1093/ps/84.6.898 PMid:15971527
21. Chung, J. Y., Kim, J. H., Ko, Y. H., Jang, I. S. (2007). Effects of dietary supplemented inorganic and organic selenium on antioxidant defense systems in the intestine, serum, liver and muscle of Korean native goats. Asian-australas. J. Anim. Sci., 20, 52–59. http://dx.doi.org/10.5713/ajas.2007.52
22. Fortier, M.E.,Audet, I., Giguère, A., Laforest, J. P., Bilodeau, J. F., Quesnel, H., Matte, J. J. (2011). Effect of dietary organic and inorganic selenium on antioxidant status, embryo development, and reproductive performance in hyperovulatory first-parity gilts. J. Anim. Sci., 90, 231-240. http://dx.doi.org/10.2527/jas.2010-3340 PMid:21890500
Copyright
© 2014 Abadjieva D. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Acknowledgement
The investigations were supported by the projects № DKOF7RP02/17 under the auspices of the Ministry of Education and Science of Bulgaria. This work was supported by the grant № BG051PO001-3.3.06-0059, financed by the European Social Fund and Operational Programme Human Resources Development (2007 – 2013) and co-financed by the Bulgarian Ministry of Education and Science.
Conflict of Interest Statement
The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.
Citation Information
Macedonian Veterinary Review. Volume 37, Issue 2, Pages 165-170, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.14432/j.macvetrev.2014.08.021, 2014
10.14432/j.macvetrev.2014.08.021
