Original Scientific Article
PCR assay with host specific internal control for Staphylococcus aureus from bovine milk samples
Zafer Cantekin
*
,
Yasar Ergun
,
Hasan Solmaz
,
Gamze Özge Özmen
,
Melek Demir
,
Radhwane Saidi
Received: 13 November 2014
Received in revised form: 13 January 2015
Accepted: 20 January 2015
Available Online First: 27 January 2015
Published on: 15 March 2015
Correspondence: Zafer Cantekin, zcantekin@hotmail.com
Abstract
Staphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in the diagnosis of S. aureus directly from milk samples instead of classical culture. However, false-negative results may occur in the polymerase chain reaction analysis performed directly from clinical material. For the purpose of disclosing the false negative results, the use of internal amplification controls can be beneficial. Therefore, in this study a new polymerase chain reaction technique with host specific internal amplification control was developed by optimizing S. aureus-specific primers in combination with bovine specific primers. The effectiveness of the developed technique in this study was attempted in milk samples from bovine subclinical mastitis. This technique has the potential to detect S. aureus from bovine milk samples or dairy products.
Keywords: bovine milk, internal control, polymerase chain reaction, Staphylococcus aureus
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Copyright
© 2015 Cantekin Z. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Conflict of Interest Statement
The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.
Citation Information
Macedonian Veterinary Review. Volume 38, Issue 1, Pages 97-100, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.14432/j.macvetrev.2015.01.038, 2015
10.14432/j.macvetrev.2015.01.038
