Macedonian Veterinary Review

Original Scientific Article

Canine adipose-derived stem cell aggregates as a viable substitute to actual canine dermal papillae

Sohee Bae * ,

Jina Kim ,

Li Li ,

Aeri Lee ,

Hyunjoo Lim ,

Junemoe Jeong ,

Seung Hoon Lee ,

Oh-kyeong Kweon ,

Wan Hee Kim

Mac Vet Rev 2015; 38 (2): 167 - 173

10.14432/j.macvetrev.2015.05.043

Received: 28 February 2015

Received in revised form: 20 April 2015

Accepted: 24 April 2015

Available Online First: 04 May 2015

Published on: 15 October 2015

Correspondence: Sohee Bae,

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Abstract

Hair loss is a major dermatological disease in veterinary and human medicine. Active studies on hair regeneration with mesenchymal stem cells have been performed in an effort to solve the limitations of conservative treatments in human medicine. Our understanding of the canine hair follicle (HF), considering a useful model for the study of the human alopecia, is limited. This study was designed to broaden our understanding of canine dermal papilla (DP), and to reconstruct dermal papilla-like tissue (DPLT) using canine adipose-derived mesenchymal stem cells (AD-MSCs), as an alternative to actual DP. We cultured canine DPs, observed their culture patterns and compared their expression level of DP-related genes and proteins with those of DPLTs by performing RT-PCR analysis and Western blotting. Canine dermal papilla cells (DPCs) showed multilayer culture patterns with pseudo-papillae. Reconstruction of DPLTs was performed successfully. Not only were they morphologically similar to actual DPs, but we also observed similarities between DPCs and DPLTs in molecular characteristics. These findings suggested that DPLT was a viable substitute for DP. This study will not only be helpful for understanding the morphological and molecular characteristics of canine DPCs, but may also serve as a basis for understanding human hair follicle biology and potential therapeutic strategies for alopecia.

Keywords: dog, dermal papillae, dermal papillae-like tissue, adipose-derived mesenchymal stem cells, hair regeneration

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Acknowledgment

This research was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (2013-011357).

Copyright

© 2015 Bae S. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non Commercial License (http://creativecommons.org), which permits unrestricted non-commercial use, distribution, and reproduction in any medium provided the original work is properly cited.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 38, Issue 2, Pages 167-173, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.14432/j.macvetrev.2015.05.043, 2015