Original Scientific Article
Phenotypic and genotypic characteristics of enterocin producing enterococci against pathogenic bacteria
Sandra Mojsova
*
,
Kiril Krstevski
,
Igor Dzadzovski
,
Zagorka Popova
,
Pavle Sekulovski
Abstract
The study investigated the antimicrobial activity of 13 enterococcal strains (E. faecalis -8, E. faecium-2, E. hirae-2, E. spp.-1) isolated from our traditional cheeses against pathogen microorganisms. Also, it includes the detection of the following enterocin structural genes: enterocin A, enterocin B, enterocin P, enterocin L50A/B, bacteriocin 31, enterocin AS48, enterocin Q, enterocin EJ97 and cytolysin by using PCR method. All isolates inhibited growth of L. monocytogenes and L.innocua. One isolate had a broader antimicrobial activity. None of the isolates showed inhibitory activity against S. enteritidis, E. coli and Y. enterocolitica. The genes enterocin P, cytolysin and enterocin A were the most frequently detected structural genes among the PCR positive strains. No amplification was obtained in two strains E. faecalis-25 and E. faecalis-86. Three different genes were identified in some strains. With the exclusion of strains possessing a virulence factor, such as cytolysin, producers of more than one enterocins could be of a great technological potential as protective cultures in the cheese industry.
Keywords: traditional cheese, enterococci, enterocins, antimicrobial activity
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Copyright
© 2015 Mojsova S. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non Commercial License (http://creativecommons.org), which permits unrestricted non-commercial use, distribution, and reproduction in any medium provided the original work is properly cited.
Conflict of Interest Statement
The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.
Citation Information
Macedonian Veterinary Review. Volume 38, Issue 2, Pages 209-216, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.14432/j.macvetrev.2015.08.052, 2015
10.14432/j.macvetrev.2015.08.052
