Mac Vet Rev 2016; 39 (1): 15 - 22
10.1515/macvetrev-2015-0060
Received: 21 May 2015
Received in revised form: 15 July 2015
Accepted: 02 September 2015
Available Online First: 04 November 2015
Published on: 15 March 2016
In this study we have devoloped and validated a confirmatory analysis method for nitrofuran metabolites, which is in accordance with European Commission Decision 2002/657/EC requirements. Nitrofuran metabolites in honey, milk, poultry meat and fish samples were acidic hydrolised followed by derivatisation with nitrobenzaldehyde and liquid-liquid extracted with ethylacetate. The quantitative and confirmative determination of nitrofuran metbolites was performed by liquid chromatography/electrospray ionisation tandem mass spectrometry (LC/ESI-MS/MS) in the positive ion mode. In-house method validation was performed and reported data of validation (specificity, linearity, recovery, CCα and CCβ). The advantage of this method is that it avoids the use of clean-up by Solid-Phase Extraction (SPE). Furthermore, low levels of nitrofuran metabolites are detectable and quantitatively confirmed at a rapid rate in all samples.
Keywords: fish, honey, LC-MS/MS, milk, nitrofuran metabolites, poultry meat
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