Original Scientific Article
Comparison of PUFA profiles in the blood and in follicular fluid and its association with follicular dynamics after PGF2α induced luteolysis in dairy cows
Branko Atanasov ,
Miel Hostens ,
Zehra Hajrulai-Musliu ,
Risto Uzunov ,
Nikola Adamov ,
Filip Davkov ,
Romel Velev ,
Geert Opsomer ,
Toni Dovenski *

Mac Vet Rev 2016; 39 (2): 175 - 183

10.1515/macvetrev-2016-0083

Received: 22 January 2016

Received in revised form: 25 April 2016

Accepted: 30 April 2016

Available Online First: 05 May 2016

Published on: 15 October 2016

Correspondence: Toni Dovenski, dovenski@fvm.ukim.edu.mk
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Abstract

The objectives of the present study were to examine the fatty acid (FA) profiles in serum and in the follicular fluid (FF) and the association between polyunsaturated fatty acid level (PUFA) and follicular growth dynamics following induced luteolysis in dairy cows. A total of 29 dairy cows (CL>25mm, follicle≈15mm) at d0 (start of the experiment) were submitted to ultrasound guided transvaginal follicular aspiration for FF collection from the largest follicle and were injected with 500 μg of cloprostenol. The cows were subdivided into Group A1 (n=11) and Group A2 (n=8) resuming follicular growth either from a secondary follicle less than or larger than 8.5mm, respectively, present at the moment of aspiration and Group A0 (n=10) not resuming follicular growth. Follicular development was monitored daily by ultrasonography until the next dominant follicle reached ≈15mm and was subsequently punctured in Group A1 and A2 (d1). Serum and FF samples for FA determination were taken at d0 from all cows and at d1 in Group A1 and A2. No differences were observed between the FA profile in serum nor in FF between sampling days. Regarding the PUFA levels, the serum linoleic acid (C18:2n6) levels at d0 and d1 were significantly higher than in FF, while alpha linolenic acid (C18:3n3) was lower in the serum than in FF, both at d0 and d1. At d0, a tendency for negative correlation between serum and the FF C18:2n6 with subsequent daily follicular growth rate was observed, while, at d1 there was a strong negative correlation between the serum C18:2n6 and daily growth rate (r=-0.71; p=0.0006). The present study revealed similarities of the FA profiles in the serum and in the FF and association between serum and FF PUFA content with the follicular dynamics after induced luteolysis.

Keywords: polyunsaturated fatty acids, ultrasound guided transvaginal follicular aspiration, cows, follicular growth

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Copyright

© 2016 Atanasov B. This is an open-access article publishedunder the terms of the Creative Commons Attribution License whichpermits unrestricted use, distribution, and reproduction in any medium,provided the original author and source are credited.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 39, Issue 2, Pages 175-183, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.1515/macvetrev-2016-0083, 2016

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.