Original Scientific Article
The influence of interspecies somatic cell nuclear transfer on epigenetic enzymes transcription in early embryos
Martin Morovic ,
Matej Murin ,
Frantisek Strejcek ,
Michal Benc ,
Dusan Paál ,
Olga Østrup ,
Heiner Niemann ,
Lazo Pendovski ,
Jozef Laurincik *

Mac Vet Rev 2016; 39 (2): 209 - 217

10.1515/macvetrev-2016-0085

Received: 21 March 2016

Received in revised form: 06 May 2016

Accepted: 08 June 2016

Available Online First: 24 June 2016

Published on: 15 October 2016

Correspondence: Jozef Laurincik, laurincik@gmail.com
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Abstract

One of the main reason for the incorrect development of embryos derived from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic nucleus. It is already known that the epigenetic enzymes transcription in oocytes and early embryos of several species including bovine and porcine zygotes is species-dependent process and the incomplete DNA methylation correlates with the nuclear transfer failure rate in mammals. In this study the transcription of DNA methyltransferase 1 and 3a (DNMT1, DNMT3a) genes in early embryonic stages of interspecies (bovine, porcine) nuclear transfer embryos (iSCNT) by RT-PCR were analyzed. Coming out from the diverse timing of embryonic genome activation (EGA) in porcine and bovine preimplantation embryos, the intense effect of ooplasm on transferred somatic cell nucleus was expected. In spite of the detection of ooplasmic DNA methyltransferases, the somatic genes for DNMT1 and DNMT3a enzymes were not expressed and the development of intergeneric embryos stopped at the 4-cell stage. Our results indicate that the epigenetic reprogramming during early mammalian development is strongly infl uenced by the ooplasmic environment.

Keywords: embryo, intergeneric nuclear transfer, DNMT1 and DMT3a gene expression, species

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Copyright

© 2016 Morovic M. This is an open-access article publishedunder the terms of the Creative Commons Attribution License whichpermits unrestricted use, distribution, and reproduction in any medium,provided the original author and source are credited.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 39, Issue 2, Pages 209-217, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.1515/macvetrev-2016-0085, 2016

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.