Original Scientific Article
The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. avium subsp. hominissuis
Vladimir Polaček
*
,
Dejan Vidanović
,
Biljana Božić
,
Žolt Beckei
,
Ivana Vučićević
,
Jasna Prodanov-Radulović
,
Sanja Aleksić-Kovacević
Received: 13 October 2016
Received in revised form: 17 November 2017
Accepted: 23 November 2017
Available Online First: 09 December 2017
Published on: 15 March 2018
Correspondence: Vladimir Polaček, vlade@niv.ns.ac.rs
Abstract
The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.
Keywords: Mycobacterium avium subsp. hominissuis, granuloma, myofibroblast, TGF-β1, TGF-β1RI
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Copyright
©2017 Polaček V. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Acknowledgement
We thank Tone Bjordal Johansen and Angelika Agdeinstain from the Norwegian Veterinary Institute for their help with real time PCR diagnostics and Danka Vukasinovic for helping translate the article. The research was partly conducted within the project of the Ministry of Education and Science of the Republic of Serbia, under the registration number TR31071, TR31084 and III46002.
Conflict of Interest Statement
The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.
Citation Information
Macedonian Veterinary Review. Volume 41, Issue 1, Pages 47-53, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.1515/macvetrev-2017-0030, 2018
10.1515/macvetrev-2017-0030
