Original Scientific Article
The evaluation of Brucella spp. isolation rates in ruminant abortion cases by using different selective media
Mustafa Sencer Karagul
*
,
Serkan Ikiz
Received: 06 March 2018
Received in revised form: 12 May 2018
Accepted: 04 July 2018
Available Online First: 16 September 2018
Published on: 15 October 2018
Correspondence: Mustafa Sencer Karagul, msencerk@hotmail.com
Abstract
The aim of this study is to evaluate the success of Brucella spp. isolation in ruminant abortion cases by using different selective media. To this end, 58 samples from ruminant abortion cases were utilized. 4 selective media; namely, Farrell Medium (FM), CITA Medium (CM), Modified Thayer Martin (MTM) and Jones & Morgan (JM) were preferred for isolation. In addition to these, one medium with antibiotics was used to extend the range of the results. Suspensions prepared from organ and fetal stomach contents were inoculated to media plates and incubated at 37Co for 5-8 days in 5-10% CO2 condition. Conventional biotyping method was used to identify Brucella isolates within the level of species and biovar. MTM (67.2%) and Farrell (65.5%) outperformed the other media with regards to isolation rate. However, regarding the inhibition ability against contaminant microrganisms, Farrell (86.2%) and CITA (72%) have the highest and second highest percentages respectively. The media’s inhibition ability was examined in the samples in which Brucella spp. isolation occurred to be able to investigate the correlations between isolation and inhibition. Lower isolation percentage was observed in the samples in which the media displayed the lowest inhibition ability against contaminants. In this context, using two different selective media with high inhibition ability against contaminants may be recommended to enhance the isolation rate. Moreover, the components stimulating the growth of Brucella strains might be added to the media to obtain better results.
Keywords: Biovar, Brucella spp., inhibition, isolation, selective medium
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Copyright
© 2018 Karagul M.S. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Acknowledgement
This article was originated from part of a PhD thesis by Mustafa Sencer Karagul. The research was approved by the Local Ethics Committee for Animal Experiments, Istanbul University, Istanbul, Turkey (Ethics Committee Decision No: 2014-84).
Conflict of Interest Statement
The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.
Citation Information
Macedonian Veterinary Review. Volume 41, Issue 2, Pages 177-186, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.2478/macvetrev-2018-0024, 2018
10.2478/macvetrev-2018-0024
