Original Scientific Article
Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts
Nora Mimoune
*
,
Rachid Kaidi
,
Mohammed Hocine Benaissa
,
Mohamed Wail Bahouh
,
Ratiba Baazizi
,
Mohamed Yassine Azzouz
Received: 02 March 2018
Received in revised form: 12 July 2018
Accepted: 18 September 2018
Available Online First: 30 December 2018
Published on: 15 March 2019
Correspondence: Nora Mimoune, nora.mimoune@gmail.com
Abstract
The aim of this study was to carry out the metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts (OC). After slaughtering, blood samples and follicular fluids from normal and cystic animals were collected and assayed using commercial kits to determine the concentrations of metabolites (glucose, total protein, total cholesterol, cortisol, triglycerides, urea, creatinine and insulin) and the liver enzymes activity. Data showed that OC were characterized by low levels of glucose, total protein, cholesterol and cortisol in cystic fluid, while urea concentrations were high compared to normal follicular fluid (P<0.001). On the other hand, serum assays of cystic animals revealed very low values of insulin and urea, whereas cortisol levels were relatively high in comparison with the serum of normal cows (P<0.001). Significant correlations between the serum and follicular fluid concentrations of normal cows were found for glucose (r=0.49), total cholesterol (r=0.31), cortisol (r=0.38) and total protein (r=0.63). The highest correlation was found for urea (r=0.86). On contrary, weak correlations were observed between metabolites concentrations in cystic fluid and in serum for normal and cystic cows. In conclusion, OC grow and persist in a metabolic environment, which differs from follicular fluid to blood. These changes may act together and/or separately to ensure the continuous development of OC. To understand a part of the mechanism, the authors propose a deep study about blood-follicle-barrier.
Keywords: ovarian cyst, follicular fluid, serum, metabolic profile
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Copyright
© 2018 Mimoune N. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Acknowledgment
The authors are grateful to the staff of Nuclear Research Laboratory, Pathological Anatomy Laboratory of Douera (Algiers, Algeria) for their kind assistance during all analyses.
Conflict of Interest Statement
The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.
Citation Information
Macedonian Veterinary Review. Volume 42, Issue 1, Pages 51-59, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.2478/macvetrev-2018-0030, 2018
10.2478/macvetrev-2018-0030
