Original Scientific Article
Homologous seminal plasma and glutathione promote pre-capacitation motility and structural stability of cryopreserved ram spermatozoa
Martin Nikolovski*,
Monika Dovenska,
Ksenija Ilievska,
Nikola Adamov,
Branko Atanasov,
Miroslav Radeski,
Daniela Kirovski,
Vladimir Petkov,
Toni Dovenski

Mac Vet Rev 2019; 42 (2): 169 - 179

10.2478/macvetrev-2019-0022

Received: 01 February 2019

Received in revised form: 24 July 2019

Accepted: 13 August 2019

Available Online First: 05 September 2019

Published on: 15 October 2019

Correspondence: Martin Nikolovski, nikolovski_m@fvm.ukim.edu.mk

Abstract

Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results in retaining structural integrity and progressive motility in post-thawed ram spermatozoa. The aims of this research were: (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. Quantitatively and qualitatively, homogenized and pooled ram ejaculates (N=10) were extended with one of the following extenders: control (C) – tris-based, GSH and HSP-free, experimental-1 (E1) – C + GSH 5 mM, experimental-2 (E2) – C + HSP 20 % and experimental-3 (E3) - GSH 5 mM + HSP 20 %. Following thawing, samples were taken at 0- and 3-hours from each group (n=10) and were assessed for spermatozoa viability, morphology, and motility pattern. C-0h samples yielded a spermatozoa population with low viability, altered head morphology and highly deviated motility pattern. E3-3h samples yielded spermatozoa with unaffected viability, head morphology and high progressive motility. In conclusion, E3 extender added to cryopreserved-thawed ram spermatozoa is most efficient in obtaining high viability, unaltered head morphology, and progressive motility.

Keywords: ram, ejaculates, cryopreservation, homologous seminal plasma, reduced glutathione


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Copyright

© 2019 Nikolovski M. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 42, Issue 2, Pages 169-179, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2019-0022, 2019