Original Scientific Article
Prevalence of subclinical mastitis pathogens in small dairy farms in Republic of North Macedonia
Aleksandar Janevsкi * ,
Iskra Cvetkovikj ,
Sanja Kiprijanovska ,
Aleksandar Dimovski ,
Katarina Davalieva ,
Ljupco Angelovski ,
Miroslav Kjosevski ,
Dine Mitrov

Mac Vet Rev 2020; 43 (1): 23 - 30

10.2478/macvetrev-2020-0010

Received: 20 October 2019

Received in revised form: 10 January 2020

Accepted: 13 January 2020

Available Online First: 24 January 2020

Published on: 15 March 2020

Correspondence: Aleksandar Janevsкi, aleksandar_janevski@yahoo.com
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Abstract

Subclinical mastitis is an asymptomatic udder infection distributed worldwide with enormous losses in the dairy industry. The study’s objective was to determine the presence of this pathological condition in small dairy farms in the R. of N. Macedonia and to identify the most common associated bacteria. Milk samples were obtained from 96 dairy cows (378 udder quarters) in seven dairy farms, in 3 consecutive samplings 24-72 hours apart. The samples were cultured on routine bacteriological growth media and incubated for 24-48 hours. The isolates were identified by AximaiD Plus MALDITOF MS Platform. Subclinical mastitis was found in 49 animals (51%) and 104 infected quarters (27%). The most frequent isolated bacteria on cow level were Streptococcus uberis (19.4%), Staphylococcus haemolyticus (13.4%), Staphylococcus aureus (7.4%) and Staphylococcus ssimulans (7.4%). On quarter level, the most isolated pathogen was Streptococcus uberis (35.6%) followed by Staphylococcus haemolyticus and Staphylococcus aureus (10.3% and 9.2% respectively). Subclinical mastitis was found to be highly present in the selected small dairy farms. The most prevalent bacteria identified in the dairy farms (Streptococcus uberis, Staphylococcus aureus and coagulase–negative staphylococci) indicate that poor management and udder health practices, inadequate milking procedures and lack of mastitis control strategies greatly contribute to occurrence and persistence of subclinical mastitis.

Keywords: subclinical mastitis, MALDI-TOF, bacteria, milk

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Copyright

© 2020 Janevski A. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 43, Issue 1, Pages 23-30, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2020-0010, 2020

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.