A simple and rapid LC-MS/MS method for determination of dexamethasone in bovine milk
Correspondence: Mehmet Nihat Ural, email@example.com
Received: 25 September 2019
Received in revised form: 02 January 2020
Accepted: 27 January 2020
Available Online First: 14 February 2020
Published on: 15 March 2020
This study provides insight into the development and validation of the simple, rapid and sensitive method of Liquid Chromatography-Tandem Mass Spectrometry for determining the residues of dexamethasone in bovine milk. The maximum residue limit for dexamethasone in bovine milk is 0.3 ng/ml-1. Chromatographic separation was achieved on an Agilent Proshell 120 SB:C18 (2.7μm 100x3.0 mm) column. The bovine milk was extracted with ethyl acetate, and the evaporated elution was dissolved with hexane, water and methanol, and analyzed by the LC-MS/MS method. The mobile phase A was a solution of 5 Mm ammonium formate in water, while the mobile phase B was 0.1% formic acid in acetonitrile. The calibration graphics were prepared within the range of 0.15–0.6 ng/ml−1, and a successful linearity was achieved (r ≥0.999). The limit of detection was 0.016 ng/ml−1. The decision limit (CCα) and detection capability (CCβ) were 0.34 and 0.38 ng/ml−1, respectively.
Keywords: dexamethasone, LC-MS/MS, milk
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© 2020 Ural M. N. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Conflict of Interest Statement
The authors have declared that no competing interests exist.
Macedonian Veterinary Review. Volume 43, Issue 1, Pages 69-73, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2020-0014, 2020