Original Scientific Article
Antimicrobial susceptibility profiles of Escherichia coli isolates from diarrheic dogs in Maiduguri, Borno State, Nigeria
Muhammad Mustapha * ,
Yusuf Audu ,
Kingsley Uwakwe Ezema ,
Jafar Umar Abdulkadir ,
Jallailudeen Rabana Lawal ,
Arhyel Gana Balami ,
Lawan Adamu ,
Yachilla Maryam Bukar-Kolo

Mac Vet Rev 2021; 44 (1): 47 - 53

10.2478/macvetrev-2020-0035

Received: 22 March 2020

Received in revised form: 08 November 2020

Accepted: 09 November 2020

Available Online First: 28 December 2020

Published on: 15 March 2021

Correspondence: Muhammad Mustapha, tanimuzimbos@gmail.com
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Abstract

Diarrhea caused by multidrug-resistant Escherichia coli (E. coli) is an important and common problem in companion animals, especially dogs. Moreover, these dogs may serve as a reservoir of pathogenic strains of E. coli that may cause enteric and extra-intestinal infections in humans and other animals. This study was conducted to investigate the antibiotic susceptibility pattern of E. coli isolates from diarrheic dogs in Maiduguri Metropolis, Borno State, Nigeria. In fecal samples of 200 dogs with diarrhea, 147 E. coli strains (73.5%) were isolated and characterized by the standard bacteriological techniques (culture, biochemical tests, and antimicrobial susceptibility testing). Out of the 147 positive isolates, 45, 50, and 52 were from Elkanemi Park, Magaram, and Sabon gari wards respectively. The isolates show 100% resistance to chloramphenicol, cefuroxime, and ceftriaxone, 96.6% to amoxicillin, and 95.9% to gentamicin, while all (100%) were susceptible to ciprofloxacin. All the isolates showed multiple antimicrobial resistance. The result of the current study showed that dogs in Maiduguri are important reservoirs of multidrug-resistant E. coli. Therefore, it is important to adopt and apply guidelines for the correct use of antimicrobials in small animal practice to reduce the emergence of multidrug resistance among E. coli in companion animals.

Keywords: Escherichia coli, dogs, diarrhea, antimicrobial susceptibility

References

  1. Greene, C.E. (1998). Infectious diseases of the dog and cat. In: C.E. Greene (Ed.), Enteric bacterial infections. 2nd ed. (243-245). Toronto, Ontario: WB Saunders Company.
  2. Cave, N.J., Marks, S.L., Kass, P.H., Melli, A.C., Brophy, M.A. (2002). Evaluation of a routine diagnostic fecal panel in dogs with diarrhea. J Am Vet Med Assoc. 221(1): 52-59. https://doi.org/10.2460/javma.2002.221.52 PMid:12420824
  3. Puno-Sarmiento, J., Medeiros, L., Chiconi, C., Martins, F., Pelayo, J., Rocha, S., Blanco, J., et al. (2013). Detection of diarrheagenic Escherichia coli strains isolated from dogs and cats in Brazil. Vet Microbiol. 166(3-4): 676-680. https://doi.org/10.1016/j.vetmic.2013.07.007 PMid:23932311
  4. Tajbakhsh, E., Ahmadi, P., Abedpour-Dehkordi, E., Arbab-Soleimani, N., Khamesipour, F. (2016). Biofilm formation, antimicrobial susceptibility, serogroups, and virulence genes of uropathogenic E. coli isolated from clinical samples in Iran. Antimicrob Resist Infect Control. 5, 11. https://doi.org/10.1186/s13756-016-0109-4 PMid:27042294 PMCid:PMC4818419
  5. Bien, J., Sokolova, O., Bozko, P. (2012). Role of uropathogenic Escherichia coli virulence factors in development of urinary tract infection and kidney damage. Int J Nephrol. 2021, 681473. https://doi.org/10.1155/2012/681473 PMid:22506110 PMCid:PMC3312279
  6. Shahrani, M., Dehkordi, F.S., Momtaz, H. (2014). Characterization of Escherichia coli virulence genes, pathotypes and antibiotic resistance properties in diarrheic calves in Iran. Biol Res. 47(1): 28. https://doi.org/10.1186/0717-6287-47-28 PMid:25052999 PMCid:PMC4105491
  7. Torkan, S., Khamesipour, F., Anyanwu, M.U. (2015). Detection of virulence and antibacterial resistance genes in Salmonella isolates from diarrhoeic dogs in Iran. Revue Méd Vét. 166(7-8): 221-228.
  8. Kavitha, K., Prabhakar, K., Rajendran, S., Uma, B., Sarayu, Y.L. (2010). Isolation of necrotoxigenic Escherichia coli from pediatric patients with acute diarrhea. J Med Microbiol. 59(Pt 4): 503-504. https://doi.org/10.1099/jmm.0.015016-0 PMid:20075117
  9. Guardabassi, L., Schwarz, S., Lloyd, D.H. (2004). Pet animals as reservoirs of antimicrobial-resistant bacteria. J Antimicrob Chemother. 54(2): 321-332. https://doi.org/10.1093/jac/dkh332 PMid:15254022
  10. Abatcha, M.G., Zunita, Z., Gurmeet, D.K., Thong, K.L. (2014). Occurrence of antibiotic resistant Salmonella isolated from dogs in Klang Valley, Malaysia. MJM. 10(3): 219-224. https://doi.org/10.21161/mjm.58213
  11. Ewers, C., Bethe, A., Semmler, T., Guenther, S., Wieller, L.H. (2012). Extended-spectrum β-lactamase-producing and AmpC-producing Escherichia coli from livestock and companion animals, and their putative impact on public health: a global perspective. Clin Microbiol Infect. 18(7): 646-655. https://doi.org/10.1111/j.1469-0691.2012.03850.x PMid:22519858
  12. Shaheen, B.W., Nayak, R., Foley, S.L., Kweon, O., Deck, J., Park, M., Rafii, F., Boothe, D.M. (2011). Molecular characterization of resistance to extended-spectrum cephalosporins in clinical Escherichia coli isolates from companion animals in the United States. Antimicrob Agents Chemother. 55(12): 5666-5675. https://doi.org/10.1128/AAC.00656-11 PMid:21947397 PMCid:PMC3232758
  13. Torkan, S., Bahadoranian, M.A., Khamesipourc, F., Anyanwu, M.U. (2016). Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran. Arch Med Vet. 48(2): 181-190. https://doi.org/10.4067/S0301-732X2016000200008
  14. Morato, E.P., Leomil, L., Beutin, L., Krause, G., Moura, R.A., Pestana de Castro, A.F. (2009). Domestic cats constitute a natural reservoir of human enteropathogenic Escherichia coli types. Zoonoses Pub Health. 56(5): 229-237. https://doi.org/10.1111/j.1863-2378.2008.01190.x PMid:19068073
  15. Pereira de Almeida, P.M., Rodrigues Arais, L. Costa Andrade, J.R., Rondon Barreto Prado, E.H., Irino, K., Figueiredo Cerqueira, A.M. (2012). Characterization of atypical enteropathogenic Escherichia coli (aEPEC) isolated from dogs. Vet Microbiol. 158(3-4): 420-424. https://doi.org/10.1016/j.vetmic.2012.02.021 PMid:22421111
  16. Paula, C.J.S., Marin, J.M. (2009). Multidrug-resistant Shiga toxin-producing Escherichia coli in dogs with diarrhea. Arq Bras Med Vet Zootec. 61(2): 511-514. https://doi.org/10.1590/S0102-09352009000200032
  17. Rzewuska, M., Czopowicz, M., Kizerwetter-Świda, M., Chrobak, D., Błaszczak, B., Binek, M. (2015). Multidrug resistance in Escherichia coli strains isolated from infections in dogs and cats in Poland (2007-2013). Scientific World J. 2015, 408205. https://doi.org/10.1155/2015/408205 PMid:25667937 PMCid:PMC4312638
  18. Younis, K., Baddour, M., Ibrahim, M.S. (2015). Detection of diarrheagenic Escherichia coli in pet animals and its antibiotic resistance in Alexandria Governorate. AJVS. 45(1): 113-118. https://doi.org/10.5455/ajvs.181517
  19. Abdulrahman, F.I., Akan, J.C., Chellube, Z.M., Waziri, M. (2012). Level of heavy metals in human hair and nail samples from Maiduguri Metropolis, Borno State, Nigeria. World Environment 2(4): 81-89. https://doi.org/10.5923/j.env.20120204.05
  20. Google Earth (2012). Maiduguri Google Earth 3D map. Available from: www.maplandia.com
  21. World Gazetteer (2007). Free Encyclopedia. [Internet]. Retrieved 2007-04-06. en.wikipedia.org/wiki/Borno_State
  22. Waziri, M. (2009). The geography of Borno: An overview. In: M. Waziri, A. Kagu, and K. M. Abubakar (Eds.), Issues in the Geography of Borno State World Gazetteer (pp. 6-8). Free Encyclopedia. Retrieved 2007-04-06.  
  23. Clinical and Laboratory Standards Institute (2012). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. [Internet]. Approved Standard-Ninth Edition. CLSI, Wayne, PA, USA. 32(2): M07-A9. https://www.medicaldesignandoutsourcing.com/clsi-publishes-2012-antimicrobial-susceptibility-testing-standards/
  24. Yousif, A.A., Mustafa, S.H., Mohammad, J.A. (2016). Clinical and molecular study of E. coli O157:H7 isolated from diarrheic and non-diarrheic dogs. MRVSA. 5(2): 1-10.
  25. Wang, J.Y., Wang, S.S., Yin, P.Z. (2006). Haemolytic-uraemic syndrome caused by a non-O157: H7 Escherichia coli strain in experimentally inoculated dogs. J Med Microbiol. 55, 23-29. https://doi.org/10.1099/jmm.0.46239-0 PMid:16388026
  26. Coura, F.M., Diniz, A.N., Oliveira Junior, C.A., Lage, A.P., Faria Lobato, F.C., Heinemann, M.B., Silveira Silva, R.O. (2018). Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil. Ciência Rural 48(2): e20170478. https://doi.org/10.1590/0103-8478cr20170478
  27. Hasan, M.S., Yousif, A., Alwan, M.J. (2016). Detection of virulent genes in E. coli O157:H7 isolated from puppies and adult dogs by polymerase chain reaction. Res J Vet Pract 4(1): 1-6. https://doi.org/10.14737/journal.rjvp/2016/4.1.1.6
  28. Chandran, A., Mazumder, A. (2013). Prevalence of diarrhea-associated virulence genes and genetic diversity in Escherichia coli isolates from fecal material of various animal hosts. Appl Environ Microbiol. 79(23): 7371-7380. https://doi.org/10.1128/AEM.02653-13 PMid:24056456 PMCid:PMC3837721
  29. Aslani, M.M., Salmanzadeh-Ahrahbi, S., Ahlikani, Y.M., Jafari, F., Zali, R.M., Mani, M. (2008). Molecular detection and antimicrobial resistance of diarrheagenic Esherichia coli isolated from diarrheal cases. Saudi Med J. 29(3): 388-392.
  30. Grave, K., Tanem, H. (1999). Compliance with short-term oral antibacterial drug treatment in dogs. J Small Anim Pract. 40(4): 158-162. https://doi.org/10.1111/j.1748-5827.1999.tb03781.x PMid:10340244
  31. Ben Said, L., Jouini, A., Alonso, C.A., Klibi, N., Dziri, R., Boudabous, A., Ben Slama, K., Torres, C. (2016). Characteristics of extended-spectrum β-lactamase (ESBL)- and pAmpC beta-lactamase-producing Enterobacteriaceae of water samples in Tunisia. Sci Total Environ. 550, 1103-1109. https://doi.org/10.1016/j.scitotenv.2016.01.042 PMid:26871556

Copyright

© 2020 Mustapha M. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 44, Issue 1, Pages 47-53, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2020-0035, 2021

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.