Original Scientific Article
Time-dependent testicular and epididymal damage in rabbit bucks experimentally infected with Trypanosoma brucei brucei
Adewale Ayodeji Adeyeye * ,
Musbaudeen Olayinka Alayande ,
Monsurat Bansima Adio ,
Lauratu Lawal Haliru ,
Aliyu Abdulrafiu

Mac Vet Rev 2021; 44 (2): 139 - 147

10.2478/macvetrev-2021-0018

Received: 23 October 2020

Received in revised form: 09 February 2021

Accepted: 18 March 2021

Available Online First: 19 May 2021

Published on: 15 October 2021

Correspondence: Adewale Ayodeji Adeyeye, adewale.adeyeye@udusok.edu.ng
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Abstract

This study was designed to determine the progressive pathological changes in the testis and epididymis of rabbit bucks experimentally infected with Trypanosoma brucei brucei (T. brucei). Fifteen local rabbit bucks were divided into 2 groups comprising 8 bucks in group A and 7 bucks in group B. Bucks in group A were infected intraperitoneally with approximately 1.0x106 of T. brucei while bucks in group B served as the uninfected control. The bucks were closely monitored for clinical signs of trypanosomosis from the time of infection till the end of the study. One buck from each group was humanely sacrificed on days 9, 19, 29 and 38 post-infection (pi) and its testis and epididymis were examined for pathological changes. At post mortem, no gross lesions were observed in the infected bucks except for adhesion of the testis and epididymis to the scrotum on days 29 and 38 pi. There were no histological changes in the testis and epididymis of the infected buck sacrificed on day 9 pi apart from mild depletion of the epididymal sperm reserve. The buck infected on day 19 pi had moderate testicular degeneration but the epididymis was devoid of sperm reserve. Severe testicular and epididymal degeneration was observed in the buck on days 29 and 38 pi. The testis and epididymis of bucks from the control group were normal with evidence of spermatogenic activities and full epididymal sperm reserve throughout. The study shows that infected bucks were unfit for breeding from day 19 pi.

Keywords: epididymis, rabbit buck, sperm reserve, testis, Trypanosoma brucei brucei

References

  1. Swallow, B.M. (2000). Impacts of trypanosomiasis on African agriculture. [Internet]. Food and agriculture organization of the United Nations. Rome. http://www.fao.org/3/X4755EN/x4755en.pdf
  2. Abubakar, Y.U., Oyedipe, E.O., Eduvie, L.O., Ogwu, D.O., Adeyeye, A.A. (2015). Reproduction and Trypanosoma congolense in Nigerian West African dwarf ewes: I. Effects on the estrous cycle. J Protozool Res. 25(1-2): 1-7.         
  3. Adeyeye, A.A., Ate, I.U., Lawal, A.I., Adamu, S. (2016). Effects of experimental Trypanosoma evansi infection on pregnancy in Yankasa ewes. Theriogenology 85(5): 862-869. https://doi.org/10.1016/j.theriogenology.2015.10.034 PMid:26643605   
  4. Adeyeye, A.A., Ate, I.U., Lawal, A.I., Adamu, S. (2016a). Postpartum pathology in Yankasa ewes experimentally infected with Trypanosoma evansi during pregnancy. Comp Clin Pathol. 25(3): 593-598. https://doi.org/10.1007/s00580-016-2236-y
  5. Alayande, M.O., Adeyeye, A.A., Abdulrafiu, A, Adio, M.B, Halilu, L.L. (2019). Sexual dimorphism of rabbit bucks and does experimentally infected with Trypanosoma brucei brucei. Veterinaria 68(2): 81-85.     
  6. Hurtado, O.J., Castro, P.D., Giraldo-Ríos, C. (2016). Reproductive failures associated with Trypanosoma (Duttonella) vivax. Vet Parasitol. 229, 54-59. https://doi.org/10.1016/j.vetpar.2016.09.017 PMid:27809979    
  7. Okubanjo, O.O., Sekoni, V.O., Ajanusi, O.J., Nok, A.J., Adeyeye, A.A. (2014). Testicular and epididymal pathology in Yankasa rams experimentally infected with Trypanosoma congolense. Asian Pac J Trop Dis. 4(3): 185-189. https://doi.org/10.1016/S2222-1808(14)60502-8
  8. Steverding, D. (2008). The history of African trypanosomiasis. Parasites Vectors. 1(1): 3. https://doi.org/10.1186/1756-3305-1-3 PMid:18275594 PMCid:PMC2270819      
  9. Desquesnes, M. (2004). Livestock trypanosomoses and their vectors in Latin America. [Internet]. OIE Paris, France. https://www.oie.int/doc/ged/D9818.PDF
  10. Sekoni, V.O. (1994). Reproductive disorders caused by animal trypanosomiases: A review. Theriogenology 42(4): 557-570. https://doi.org/10.1016/0093-691X(94)90373-Q
  11. Milligan, P.J.M., Baker, R.D. (1988). A model of tsetse-transmitted animal trypanosomiasis. Parasitology 96(1): 211-239. https://doi.org/10.1017/S0031182000081774 PMid:3362578              
  12. Argungu, S.Y., Bala, A.Y., Majeed, Q., Usman, M., Umar, A.A. (2015). Possible transplacental transmission of Trypanosoma evansi in experimentally infected albino rats. J Zool Biosci Res. 2(1): 23-27.        
  13. Ate, I.U., Rekwot, P.I., Nok, A.J., Tekdek, L.B., Luga, I.J. (2006). On-farm transplacental transmission of Trypanosoma vivax in a Bunaji calf in Zaria, Northern Nigeria. Niger Vet J. 27(3): 95-98. https://doi.org/10.4314/nvj.v27i3.3533
  14. Kumar, R., Kumar, S., Virmani, N., Yadav, S.C. (2015). Transplacental transmission of Trypanosoma evansi from experimentally infected donkey mare to neonatal foal. J Equine Vet Sci. 35(4): 337-341. https://doi.org/10.1016/j.jevs.2015.02.004
  15. OIE. (2013). [Internet]. Trypanosomosis- tsetse transmitted. In: Office of the international epizootic. Terrestrial manual (pp. 1-11). https://www.oie.int/doc/ged/D9818.PDF
  16. Akpavie, S.O., Ikede, B., Egbunike, G.N. (1987). Ejaculate characteristics of sheep infected with Trypanosoma brucei and T. vivax: changes caused by treatment with diminazene aceturate. Res Vet Sci. 42(1): 1-6. https://doi.org/10.1016/S0034-5288(18)30647-7
  17. Ukwueze, C.S., Anene, B.M. (2011). The effects of diabetes mellitus on the pathogenicity of trypanosomosis in rats. JVMAH. 3(3): 40-44.       
  18. Wada, Y.A., Oniye, S.J., Rekwot, P.I., Okubanjo, O.O. (2016). Single and mixed interaction of experimental Trypanosoma brucei brucei and Trypanosoma evansi on the semen collection reaction time and spermatozoa morphology of Yankasa rams. J Adv Vet Anim Res. 3(4): 360-367. https://doi.org/10.5455/javar.2016.c174
  19. Wada, Y.A., Oniye, S.J., Rekwot, P.I., Okubanjo, O.O. (2016). Testicular pathology, gonadal and epididymal sperm reserves of Yankasa rams infected with experimental Trypanosoma brucei brucei and Trypanosoma evansi. Vet World. 9(7): 759-765. https://doi.org/10.14202/vetworld.2016.759-765 PMid:27536039 PMCid:PMC4983129   
  20. Amin, Y.A., Noseer, E.A., Fouad, S.S., Ali, R.A., Mahmoud, H.Y. (2020). Changes of reproductive indices of the testis due to Trypanosoma evansi infection in dromedary bulls (Camelus dromedarius): semen picture, hormonal profile, histopathology, oxidative parameters, and hematobiochemical profile. J Adv Vet Anim Res. 7(3): 537-545. https://doi.org/10.5455/javar.2020.g451 PMid:33005681 PMCid:PMC7521820    
  21. Allam, L., Ogwu, D., Agbede, R.I., Sackey, A.K. (2012). Posterior paresis in pregnant gilts experimentally infected with Trypanosoma brucei. JVMAH. 4(3): 27-35.  
  22. Allam, L., Ogwu, D., Agbede, R.I., Sackey, A.K. (2014). Abortion and its probable cause in gilts experimentally infected with Trypanosoma brucei. J Protozool Res. 24, 26-32.     
  23. Leigh, O.O., Emikpe, B.O., Ogunsola, J.O. (2015). Histopathological changes in some reproductive and endocrine organs of Trypanosoma brucei infected West African dwarf goat does. Bulg J Vet Med. 18(1): 31-39. https://doi.org/10.15547/bjvm.831
  24. Leigh, O.O., Fayemi, O.E. (2013). The effect of experimental Trypanosoma brucei infection on hormonal changes during the oestrous cycle, pregnancy and pregnancy outcome in West Africa Dwarf does. WJAS. 5, 685-695.      
  25. Adamu, S., Fatihu, M.Y., Useh, N.M., Mamman, M., Sekoni, V.O., Esievo, K.A. (2007). Sequential testicular and epididymal damage in Zebu bulls experimentally infected with Trypanosoma vivax. Vet Parasitol. 143(1): 29-34. https://doi.org/10.1016/j.vetpar.2006.07.022 PMid:16935425    
  26. Woo, P.T.K. (1970). The haematocrit centrifuge technique for the diagnosis of African trypanosomiasis. Acta Trop. 27(4): 384-386.        
  27. Sekoni, V.O., Njoku, C.O., Kumi-Diaka, J., Saror, D.I. (1990). Pathological changes in male genitalia of cattle infected with Trypanosoma vivax and Trypanosoma congolense. Br Vet J. 146(2): 175-180. https://doi.org/10.1016/0007-1935(90)90011-Q
  28. CACC (2020). Guide to the care and use of experimental animals (Volume 1, 2nd ed.) c2020 [cited 2020 June 17]. http://www.ccac.ca/Documents/Standards/Guidelines/Experimental_Animals_Vol1.pdf
  29. Ezema, W.S., Omeke, B.C., Eze, J.I., Nwanta, J.A. (2009). Studies on the sexual behaviour, haematology and spermatogenesis of male rabbits infected with Trypanosoma brucei brucei. Comp Clin Path. 18(1): 63-68. https://doi.org/10.1007/s00580-008-0740-4
  30. Batista, J.S., Riet-Correa, F., Teixeira, M.M., Madruga, C.R., Simões, S.D., Maia, T.F. (2007). Trypanosomiasis by Trypanosoma vivax in cattle in the Brazilian semiarid: description of an outbreak and lesions in the nervous system. Vet Parasitol. 143(2): 174-181. https://doi.org/10.1016/j.vetpar.2006.08.017 PMid:16965857    
  31. Okpala, M.I., Ezeh, I.O., Obi, C.F., Ochiogu, I.S., Obidike, R.I., Ezeokonkwo, R.C. (2019). Impaired epididymal function in Trypanosoma brucei brucei and Trypanosoma congolense infected and treated albino rats. Comp Clin Path. 28(5): 1-6. https://doi.org/10.1007/s00580-019-02983-2
  32. Okubanjo, O.O., Sekoni, V.O., Ajanusi, O.J., Adeyeye, A.A. (2015). Effects of experimental Trypanosoma congolense infection on sperm morphology in Yankasa rams. Mac Vet Rev. 38(2): 203-208. https://doi.org/10.14432/j.macvetrev.2015.07.050
  33. Hermo, L., Robaire, B. (2002). Epididymal cell types and their functions. In: B. Robaire, B. Hinton (Eds.), The epididymis: from molecules to clinical practice (pp. 81-102). Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0679-9_5
  34. Victor, I., Sackey, A.K., Natala, A.J. (2012). Penile protrusion with hemorrhages and preputial inflammation in pigs experimentally infected with Trypanosoma congolense. J Anim Prod Adv. 2(6): 297-302.   
  35. Anosa, V.O., Isoun, T.T. (1980). Further observations on the testicular pathology in Trypanosoma vivax infection of sheep and goats. Res Vet Sci. 28(2): 151-160. https://doi.org/10.1016/S0034-5288(18)32738-3
  36. Ikede, B.O., Elhassan, E., Akpavie, S.O. (1988). Reproductive disorders in African trypanosomiasis: A review. Acta Trop. 45(1): 5-10.           
  37. Silva Pereira, S., Trindade, S., De Niz, M., Figueiredo, L.M. (2019). Tissue tropism in parasitic diseases. Open Biol. 9(5): 190036. https://doi.org/10.1098/rsob.190036 PMid:31088251 PMCid:PMC6544988
  38. Carvalho, T., Trindade, S., Pimenta, S., Santos, A.B., Rijo-Ferreira, F., Figueiredo, L.M. (2018). Trypanosoma brucei triggers a marked immune response in male reproductive organs. PLoS Negl Trop Dis. 12(8): e0006690. https://doi.org/10.1371/journal.pntd.0006690 PMid:30110342 PMCid:PMC6093638      
  39. Abubakar, Y.U., Oyedipe, E.O., Eduvie, L.O., Ogwu, D.O., Adeyeye, A.A. (2016). Reproduction and Trypanosoma congolense in Nigerian West African dwarf ewes: II. Genital and endocrine lesions. Afr J Cell Path. 6, 16-20.
  40. Mbaya, A.W., Nwosu, C.O., Kumshe, H.A. (2011). Genital lesions in male red fronted gazelles (Gazella rufifrons) experimentally infected with Trypanosoma brucei and the effect of melarsamine hydrochloride (Cymelarsan®) and diminazene aceturate (Berenil®) in its treatment. Theriogenology 76(4): 721-728. https://doi.org/10.1016/j.theriogenology.2011.04.004 PMid:21601916   
  41. Omeke, B.C., Igboeli, G. (2000). Disruption of spermatogenesis in boars sub-clinically infected with Trypanosoma brucei brucei. Anim Reprod Sci. 63(3-4): 197-204. https://doi.org/10.1016/S0378-4320(00)00172-X
  42. Shehu, S.A., Ibrahim, N.D., Esievo, K.A., Mohammed, G. (2006). Pathology of experimental Trypanosoma evansi infection in Savannah Brown Buck. Pak J Biol Sci. 9(3): 522-525. https://doi.org/10.3923/pjbs.2006.522.525

Copyright

© 2021 Adeyeye A.A. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 44, Issue 2, Pages 139-147, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2021-0018, 2021

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.