Original Scientific Article
Isolation rate of Campylobacter spp. and detection of virulence genes of Campylobacter jejuni across the broiler chain
Ljupco Angelovski*,
Zagorka Popova,
Katerina Blagoevska,
Sandra Mojsova,
Marija Ratkova Manovska,
Mirko Prodanov,
Dean Jankuloski,
Pavle Sekulovski

Mac Vet Rev 2021; 44 (2): 149 - 157

10.2478/macvetrev-2021-0020

Received: 16 March 2021

Received in revised form: 10 May 2021

Accepted: 17 May 2021

Available Online First: 19 June 2021

Published on: 15 October 2021

Correspondence: Ljupco Angelovski, angelovski@fvm.ukim.edu.mk

Abstract

The aim of the study was to identify the isolation rate of thermotolerant campylobacters in a small-scale broiler-meat production farm over a one-year period. The second deliverable of the study was to determine the potential virulence markers. The laboratory investigation was performed on 283 samples (cloacal swabs, caeca, carcass swabs) collected on three sampling points (farm, slaughter line, and cold storage). The isolates obtained with the conventional microbiological method were confirmed with multiplex PCR for identification of campylobacters. The presence of 10 virulence genes was analyzed in the C. jejuni isolates ( flaA, racR, virB11, dnaJ, wlaN, cadF, ciaB, cdtA, cdtB, cdtC). Out of 283 samples, 169 (59.7%) were confirmed as Campylobacter spp., 111 (39.2%) C. jejuni, and 43 (15.2%) C. coli. C. jejuni was the most prevalent in all sampling points. Campylobacter spp. showed a characteristically seasonal prevalence with the highest isolation rate during the warmer period of the year. We detected the cadF and ciaB genes in all C. jejuni isolates. The flaA gene was present in 50% of the examined strains. The cdt genes (cdtA, cdtB, and cdtC) were confirmed in 52.8%, 52.8%, and 47.2% of the C. jejuni strains, respectively. C. jejuni showed 15 profiles of virulence patterns with four predominant profiles.

Keywords: isolation rate, Campylobacter spp., virulence, Campylobacter jejuni, broiler chain


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Copyright

© 2021 Angelovski Lj. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

Competing Interests: The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 44, Issue 2, Pages 149-157, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2021-0020, 2021