Original Scientific Article
The microscopic detection of animal proteins in animal feed regarding bovine spongiform encephalopathy
Radmila Crceva Nikolovska*,
Aleksandra Angeleska,
Srecko Gjorgjievski,
Katerina Blagoevska,
Riste Uzunov,
Vasilka Poposka Trenevska

Mac Vet Rev 2021; 44 (2): 169 - 178


Received: 03 April 2021

Received in revised form: 21 June 2021

Accepted: 26 June 2021

Available Online First: 05 July 2021

Published on: 15 October 2021

Correspondence: Radmila Crceva Nikolovska, rnikolovska@fvm.ukim.edu.mk


Due to the actuality of spongiform encephalopathies and their proven spreading by means of animal feed containing meat and bone meal, the description and measurement of osteocytic lacunae contributes to more easily distinguish bone fragments in meat and bone meal. Transmissible spongiform encephalopathies (TSEs) have attracted a lot of attention, especially after 1986, when the first case of BSE (bovine spongiform encephalopathy) was detected. Since the outbreak of spongiform encephalopathy (BSE), the use of animal protein including bone meal as an ingredient in animal feed has been controlled by several regulations including Regulation (EC) 999/2001, Regulation (EC) 1774/2002, and Regulation (EC) 1234/2003. The classical microscopic method is the only official method for detecting animal protein in animal feed in the European Union (Commission Regulation (EC) 152/2009). By applying the microscopic method to the animal feed samples, we performed detection in order to determine the presence of animal proteins that originate from mammals and fish. The microscopic analysis of all 421 samples, of which 115 were raw materials for the production of animal feed, 230 were concentrates for ruminant nutrition and 76 were concentrates for non-ruminant nutrition (32 concentrates for laying hens and 44 concentrates for pigs), did not provide positive results, that is, no remains of animal tissues of mammalian origin were found in any specimen. Whereas in 10 out of 32 (31.25%) concentrates intended for non-ruminant nutrition (laying hens), pieces of fish tissue were found. In these samples, we usually detected the presence of fish bones, gills and scales.

Keywords: animal proteins in animal feed, microscopy, decision support system, ARIES


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© 2021 Crceva Nikolovska R. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 44, Issue 2, Pages 169-178, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2021-0021, 2021