Original Scientific Article
The microscopic detection of animal proteins in animal feed regarding bovine spongiform encephalopathy
Radmila Crceva Nikolovska * ,
Aleksandra Angeleska ,
Srecko Gjorgjievski ,
Katerina Blagoevska ,
Riste Uzunov ,
Vasilka Poposka Trenevska

Mac Vet Rev 2021; 44 (2): 169 - 178

10.2478/macvetrev-2021-0021

Received: 03 April 2021

Received in revised form: 21 June 2021

Accepted: 26 June 2021

Available Online First: 05 July 2021

Published on: 15 October 2021

Correspondence: Radmila Crceva Nikolovska, rnikolovska@fvm.ukim.edu.mk
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Abstract

Due to the actuality of spongiform encephalopathies and their proven spreading by means of animal feed containing meat and bone meal, the description and measurement of osteocytic lacunae contributes to more easily distinguish bone fragments in meat and bone meal. Transmissible spongiform encephalopathies (TSEs) have attracted a lot of attention, especially after 1986, when the first case of BSE (bovine spongiform encephalopathy) was detected. Since the outbreak of spongiform encephalopathy (BSE), the use of animal protein including bone meal as an ingredient in animal feed has been controlled by several regulations including Regulation (EC) 999/2001, Regulation (EC) 1774/2002, and Regulation (EC) 1234/2003. The classical microscopic method is the only official method for detecting animal protein in animal feed in the European Union (Commission Regulation (EC) 152/2009). By applying the microscopic method to the animal feed samples, we performed detection in order to determine the presence of animal proteins that originate from mammals and fish. The microscopic analysis of all 421 samples, of which 115 were raw materials for the production of animal feed, 230 were concentrates for ruminant nutrition and 76 were concentrates for non-ruminant nutrition (32 concentrates for laying hens and 44 concentrates for pigs), did not provide positive results, that is, no remains of animal tissues of mammalian origin were found in any specimen. Whereas in 10 out of 32 (31.25%) concentrates intended for non-ruminant nutrition (laying hens), pieces of fish tissue were found. In these samples, we usually detected the presence of fish bones, gills and scales.

Keywords: animal proteins in animal feed, microscopy, decision support system, ARIES

References

  1. Hendriks, W.H., Butts, C.A., Thomas, V.D., James, K.A.C., Morel, P.C.A., Verstegen, M.W.A. (2002). Nutritional quality and variation of meat and bone meal. Asian-Aust. J. Anim. Sci. 15(10): 1507-1516. https://doi.org/10.5713/ajas.2002.1507
  2. Rodehutscord, M., Abel, H.J., Friedt, W, Wenk, C., Flachowsky, G.,Ahlgrimm, H.J., Johnke, B., et al. (2002). Consequences of the ban of by-products from terrestrial animals in livestock feeding in Germany and the European Union: alternatives, nutrient and energy cycles, plant production, and economic aspects. Arch Tierernahr. 56, 67-91. https://doi.org/10.1080/00039420214180 PMid:12389223
  3. Budka, H., Kovacs, G. (2008). Prion diseases: From protein to cell pathology. Am J Pathol. 172(3): 555-565. https://doi.org/10.2353/ajpath.2008.070442 PMid:18245809 PMCid:PMC2258253
  4. Wells, G.A.H., Hawkins, S.A.C., Austin, A.R., Ryder, S.J., Done, S.H., Green, R.B., Dexter, I., et al. (2003). Studies of the transmissibility of the agent of bovine spongiform encephalopathy to pigs. J Gen
  5. Virol. 84, 1021-1031. https://doi.org/10.1099/vir.0.18788-0 PMid:12655106
  6. Wells, G.A.H., Konold, T., Arnold, M.E., Austin, A.R., Hawkins, S.A.C., Stack, M., Simmons, M.M., et al. (2007). Bovine spongiform encephalopathy: the effect of oral exposure dose on attack rate and incubation period in cattle. J Gen Virol. 88, 1363-1373. https://doi.org/10.1099/vir.0.82421-0 PMid:17374783
  7. Moore J., Hawkins, S.A.C., Austin, A.R., Konold, T., Green, R.B., Blamire, I. W., Dexter, I., et al. (2011). Studies of the transmissibility of the agent of bovine spongiform encephalopathy to the domestic chicken. BMC Research Notes 4, 501. https://doi.org/10.1186/1756-0500-4-501 PMid:22093239 PMCid:PMC3341577
  8. Matthews, D., Cooke, B.C. (2003). The potential of transmissible spongiform encephalopathies in non-ruminant livestock and fish. Rev Sci Tegh Off Int Epiz. 22, 283-296. https://doi.org/10.20506/rst.22.1.1393 PMid:12793786
  9. Beringue, V., Andreoletti, O., Le Dur, A., Essalmani, R., Vilotte, J., Lacroux, C., Reine, F., et al. (2007). A bovine prion acquires an epidemic bovine spongiform encephalopathy strein-like phenotype on interspecies transmission. J Neurosci. 27, 6965-6971. https://doi.org/10.1523/JNEUROSCI.0693-07.2007 PMid:17596445 PMCid:PMC6672218
  10. Gizzi, G., Von Holst, C., Baeten, V., Berben, G., Van Raamsdonk, L. (2003). An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongioform encephalopathy. Rev Sci Tech Off Int Epiz. 22 (1): 311- 331. https://doi.org/10.20506/rst.22.1.1399 PMid:12793788
  11. Domenis, L., Squadrone, S., Marchis, D., Abete, M. C. (2009). Osteocyte lacunae features in different chicken bones. Biotechnol Agron Soc Environ. 13, 29-32.
  12. Sakač, M., Filipović, S., Borojević, Č., Ristić, M., Kormanjoš, Š. (2004). Influence of еxtrusion of total glucosinolate content of rapeseed. Proceedings of the 45th Consulting, Production and Processing of Oilseeds 45, 133-140.
  13. Kwiatek, K., Weiner, A. (2003). Presence of processed animal protein in eedingstuffs for ruminants. Bull Vet Inst Pulawy. 47, 515-521. 13. Hahn, H. (1999). Animal meal: production and determination in feedstuffs and the origin of bovine spongiform encephalopathy. Naturwissenschaften 86, 62-70. https://doi.org/10.1007/s001140050573 PMid:10084149
  14. Engling, F. P., Jörgenson, J. S., Paradies-Severin, I., Hahn, H. (2000). Evidence of animal meal in feeds. Kraftfutter/Feed Magazine 1, 14-17.
  15. Van Raamsdonk, H., Van Der Voet, L. (2003). A ring trial for the detection of animal tissue in feeds in the presence of fish meal. Rikilt Institute of Food Safety. Report 2003. 012.
  16. Gizzi, G., Von Holst, C., Baeten, V., Berben, G., Van Raamsdonk, L. (2003). Intercomparison study for the determination of processed animal proteins including meat and bone meal in animal feed. Final Report of 16 May 2003, Institute for Reference Materials and Measurements, European Commission, Ispra, Italy. Administrative arrangement No B5-1000/02/000483
  17. Van Raamsdonk, WD L., Vancutsem, J., Zegers, J., Frick, G., Jorgenson, J., Pinckaers, V., Bosch, J., Paradies-Severin, I. (2004). The microscopic detection of animal proteins in feeds, Biotechnol Agron Soc Environ. 8(4): 241-247.
  18. European Commission: Directorate-general for health and consumers [Internet]. The TSE Roadmap 2. A Strategy paper on Transmissible Spongiform Encephalopathies for 2010-2015. [cited 2010 July 16]. https://ec.europa.eu/food/system/files/2016-10/biosafety_food-borne-disease_tse_road-map2.pdf

Copyright

© 2021 Crceva Nikolovska R. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 44, Issue 2, Pages 169-178, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2021-0021, 2021

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.