Original Scientific Article
The effect of alkaline water and sodium ascorbate on glucose and cortisol levels during acute hyperthermic stress in white laboratory rats
Valdrina Ajeti*,
Slagjana Brsakoska,
Vasilka Rendjova,
Marija Angelovski,
Icko Gjorgoski

Mac Vet Rev 2021; 44 (2): 179 - 185


Received: 12 May 2021

Received in revised form: 30 June 2021

Accepted: 14 July 2021

Available Online First: 16 August 2021

Published on: 15 October 2021

Correspondence: Valdrina Ajeti, valdrinaajetii@gmail.com


Stress can be a reason for some physiological and biological disorders in the body. The antioxidative defense system is necessary for the maintenance of redox homeostasis in the organisms. Alkaline water (AW) is in the focus of the scientific interest due to its antioxidative effect. The treatment with AW and sodium ascorbate (SA) is expected to have potential preventive effect on the organism to hyperthermic stress. The aim of this study was to investigate the effect of AW and SA on glucose and cortisol levels during acute hyperthermic stress, in white female Wistar laboratory rats. The rats were divided into three groups, each having 10 subjects. They were exposed in hyperthermic conditions (41˚C) for 80 min, in 21 consecutive days in order to induce oxidative stress. The first group received drinkable water (control group), the second AW, and the third, AW and SA. Plasma glucose levels were determined by colorimetric method. Cortisol level was measured by the enzyme-linked immunosorbent assay method (ELISA). The means were compared using the Tukey test. Differences were considered significant at a level of p<0.05. Our results showed that levels of glucose and cortisol were significantly higher in the group treated with AW on the 21st day after treatment (p<0.0001), but not on the 7th and 14th day as compared to the control group. Also, co-treatment of animals with AW and SA had significantly increased the levels of glucose and cortisol on the 21st day after treatment, indicating a synergistic effect. In conclusion, the individual action of AW or in synergism with SA caused a high protective effect on oxidative damage in white Wistar laboratory rats.

Keywords: alkaline water, sodium ascorbate, hyperthermic stress, glucose, cortisol


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© 2021 Ajeti V. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 44, Issue 2, Pages 179-185, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2021-0023, 2021