Original Scientific Article
Hypericum perforatum L. hairy root extracts – regulation of glycemic, metabolic, serum enzyme and lipid profile in STZ - induced diabetic rats
Elena Rafailovska,
Oliver Tushevski,
Sonja Gadzovska-Simic,
Suzana Dinevska-Kjovkarovska,
Biljana Miova*

Mac Vet Rev 2022; 45 (1): 5 - 15


Received: 30 August 2021

Received in revised form: 28 September 2021

Accepted: 08 October 2021

Available Online First: 23 November 2021

Published on: 15 March 2022

Correspondence: Biljana Miova, bmiova@pmf.ukim.mk


Apart from currently available therapeutics for the treatment of diabetes mellitus, much attention has been paid to discover phytochemicals from natural resources, mainly due to their low side-effects. Hypericum perforatum hairy root (HR) transformed with Agrobacterium rhizogenes A4 represent prospective experimental system enriched in xanthones, known as potent antidiabetic agents. Thus, the aim of this study was to evaluate HR extracts for their potential antihyperglycemic activity in streptozotocin (STZ)-induced diabetic rats, also compared to the effects of wild-growing Hyperici herba (HH). We conducted an acute-toxicity study, multiple dose study, and 24h blood glucose measurements after a single dose administration of HH and HR (200 mg/kg) in diabetic rats. Furthermore, we examined the effects of 14-days administration of HH and HR extracts on blood glucose levels, metabolic parameters, enzyme, and lipid status in healthy and diabetic rats. Both extracts produced a fall of about 70% in blood glucose level after 24h of administration. Two-week treatment with HH and HR induced a significant decrease (70-72%) in blood glucose levels. Moreover, we found an improvement of the dysregulated metabolic parameters (body weight, food, and water consumption and urine output). Serum enzyme (AST, ALT, and γ-GT) and lipid profile parameters (CHOL, TAG, and HDL) were also improved by both extracts. These findings might provide a new insight for managing diabetic hyperglycemia and dysregulated serum enzyme and lipid profile, using extracts from transgenic roots cultures from H. perforatum.

Keywords: hairy roots, hanthones, STZ-diabetic rats, Hypericum perforatum L., antihyperglycemic


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© 2021 Rafailovska E. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 45, Issue 1, Pages 5-15, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2021-0027, 2022