Original Scientific Article
Carbohydrate metabolism in diabetic rat’s heart – the effects of acetylsalicylic acid and heat preconditioning as HSP70 inducers
Mirsada Dervisevik ,
Suzana Dinevska- Kjovkarovska ,
Sasho Panov ,
Elena Rafailovska ,
Irena Celeska ,
Biljana Miova *

Mac Vet Rev 2022; 45 (2): 137 - 148


Received: 02 February 2022

Received in revised form: 31 May 2022

Accepted: 06 June 2022

Available Online First: 27 July 2022

Published on: 15 October 2022

Correspondence: Biljana Miova, bmiova@pmf.ukim.mk


The myocardium of diabetic subjects displays reduced HSP70 protein level and weak myocardial protection. However, the heart possesses an ability to produce heat shock proteins (HSPs) after exposure to sublethal heat stress. Acetylsalicylicacid (ASA) has the property of pharmacological induction of HSPs. We evaluated the common effects of single dose ASApretreatment, prior to heat preconditioning (HP), over carbohydrate metabolism-related enzymes and substrates in the heart of diabetic rats. Streptozotocin-diabetes caused significant decrease of HSP70 protein level, stimulation of the gluconeogenic processes and inhibition of glycolytic processes in the heart. HP-diabetic hearts have significantly higher HSP70 protein level, lower glycogen, glucose-6-phosphate content, glycogen phosphorylase and hexokinase activity, and higher glucose levels and PFK activity. ASA-pretreatment of HP-diabetic animals caused additional increase of HSP70, additional decrease of glycogen, glucose-6-phosphate, glycogen phosphorylase and hexokinase, and additional increase of glucose and PFK in the heart. In conclusion, HP is physiological inducer of HSP70 level in heart and tends to reverse carbohydrate - related disturbances in diabetic rats. ASA, given prior to HP, is a potent HSP70 co-inducer and causes additional increase of HSP70 protein level in heart. ASA, given in a combination to HP, have shown more evident protective effects against subsequent intense of stress.

Keywords: heat preconditioning, acetylsalicylic acid, streptozotocin-induced diabetes, heart, carbohydrate-related enzymes and substrates


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© 2022 Dervisevik M. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 45, Issue 2, Pages 137-148, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2022-0021, 2022