Short Communications
Use of probiotic Bacillus megaterium NCH 55 for treatment of subclinical mastitis in cows – preliminary study
Oksana Shkromada ,
Alina Pikhtirova * ,
Ewa Pecka-Kiełb ,
Oleksandr Skliar ,
Yurii Musiienko

Mac Vet Rev 2022; 45 (2): 209 - 214


Received: 17 November 2021

Received in revised form: 10 May 2022

Accepted: 29 July 2022

Available Online First: 02 September 2022

Published on: 15 October 2022

Correspondence: Alina Pikhtirova,


The development and implementation of new and safe means of treating subclinical mastitis (SM) in the production process remains a pressing problem. Our study aimed to investigate the therapeutic effect of the Bacillus megaterium NCH 55 strain on cow SM. All animals with signs of SM were fed with a basic diet with probiotic’s addition of 15 g/per day. The experiment was conveyed in 30 consecutive days. Milk and blood were collected on the 7th, 15th, and 30th day of the experiment. Daily milk yield was increased by 32.2% and somatic cell count was decreased from 290.00±32.12 to 96.80±39.03 ths/cm3. A decrease in inflammation was indicated by a decrease in POM370 (by 2.2 times) and POM430 (by 2.8 times). No significant changes in the blood serum indices of cows were observed. A probiotic’s positive effect has been established on the treatment of SM. The probiotic B. megaterium NСH 55 can be considered as an alternative to antibiotics and other chemotherapeutic drugs for treating SM in cows.

Keywords: cow, mastitis, milk, probiotics, Bacillus megaterium


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© 2022 Shkromada O. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 45, Issue 2, Pages 209-214, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2022-0023, 2022