Original Scientific Article
Toxic effects of sodium lauryl sulfate on antioxidant defense system and DNA damage in fish primary hepatocyte cultures
Aslı Ç. Yeltekin * ,
Ahmet R. Oğuz

Mac Vet Rev 2022; 45 (2): 169 - 175


Received: 22 September 2021

Received in revised form: 01 September 2022

Accepted: 05 September 2022

Available Online First: 15 September 2022

Published on: 15 October 2022

Correspondence: Aslı Ç. Yeltekin, aslicyeltekin@gmail.com


Synthetic detergents which have a major role in environmental pollution accumulate over time and reach levels that harm nature. The surfactants which are abundantly used as cleaning components are discharged into the Van Lake with the sewage water. These chemicals accumulating in the lake may reach a level that could affect the only fish species of the lake, the Van fish. This study aimed to determine the antioxidant levels of Van fish hepatocyte cell culture medium treated with sodium lauryl sulphate (SLS) and to assess the DNA damage. The effect of SLS was assessed by its dose (1x10-5, 1x10-6, 1x10-7 M) and treatment time (24 h, 48 h). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and DNA damage (8-OHdG) were determined in the SLS hepatocyte culture. SOD and GSH-Px were higher on 24 h and 48 h compared to the control group. A significant increase was observed in CAT level in the first 24 h, especially in 1x10-6 and 1x10-5 M concentration. At 48 h, it was observed that the CAT level decreased significantly as the concentration increased. It was determined that MDA and 8-OHdG levels increased depending on concentration and time. In conclusion, different concentrations of SLS affected antioxidant levels in the primary hepatocyte culture of Van Fish and were found to cause an increase in the levels of MDA and 8-OHdG.

Keywords: SLS, fish, DNA damage, antioxidants, MDA


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© 2022 Yeltekin A.Ç. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 45, Issue 2, Pages 169-175, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2022-0027, 2022