Original Scientific Article
Profiling Mycoplasma hyopneumoniae infection in commercial pig farms using serology and lung lesions assessment
Branko Angjelovski * ,
Clara Marin Orenga ,
Aleksandar Janevski ,
Aleksandar Dodovski ,
Radisa Prodanovic ,
Jovan Bojkovski

Mac Vet Rev 2023; 46 (2): 129 - 135

10.2478/macvetrev-2023-0018

Received: 01 February 2023

Received in revised form: 29 March 2023

Accepted: 03 April 2023

Available Online First: 28 April 2023

Published on: 15 October 2023

Correspondence: Branko Angjelovski, brankoa@fvm.ukim.edu.mk
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Abstract

Mycoplasma hyopneumoniae (Mhyo) is primary pathogen involved in porcine respiratory diseases complex (PRDC). The objective of this study was to evaluate Mycoplasma hyopneumoniae (Mhyo) infection in five commercial pig farms by using serology and lung lesion scoring at slaughter. Five Macedonian pig herds with a history of respiratory diseases were included in the study. Blood samples were taken from pigs at 6, 10, 14, 18, and 22 weeks of age. Ten animals per age group from each farm were sampled and tested for antibodies to Mhyo. At abattoir, 50 lungs per farm were scored for lesions associated with enzootic pneumonia (EP). All farms were seropositive to Mhyo. Higher seroprevalence to Mhyo was observed in grower and finisher pigs, while significant difference (p<0.001) was detected among farms in 10-, 14-, and 22-week-old pigs. Enzootic pneumonia-like lesions were detected in 91.2% of all tested lungs with range of 82 to 98% on farm level. Mean lung lesion score (LLS) obtained for all farms was 11.5 (8.04–14.4). Significant difference for LLS and significantly higher percentage of severe LLS grade (>10) were found among some of the farms (p<0.001). In conclusion, high seroprevalence to Mhyo in finishing pigs in most of the farms was most likely due to vaccination. Farms with higher seroprevalence to Mhyo obtained lower LLS. Serology monitoring of different pig categories and lung assessment at slaughterhouse is a practical tool for assessing vaccine efficacy of Mhyo in pig farms.

Keywords: lung lesion, Mycoplasma hyopneumoniae, pig, serology

References

1. Van Alstine, W.G. (2006). Respiratory system. In: J.J. Zimmerman, L.A. Karriker, A. Ramirez, K.J. Schwartz, G.W. Stevenson (Eds.), Diseases of swine, 10th ed. (pp. 348-362). Ames, IA, WileyBlackwell Publishing
2. Fraile, L., Alegre, A., LopezJimenez, R., Nofrarias, M., Segales, J. (2010). Risk factors associated with pleuritis and cranioventral pulmonary consolidation in slaughter aged pigs. Vet J. 184(3): 326-333. https://doi.org/10.1016/j.tvjl.2009.03.029 PMid:19527939
3. Yoeger, M.J., Van Alstine, W.G. (2019). Respiratory system. In: J.J. Zimmerman, L.A. Karriker, A. Ramirez, K.J. Schwartz, G.W. Stevenson, Z. Jianqiang (Eds.), Diseases of swine, 11th ed. (pp.393-407). New York: Wiley-Blackwell https://doi.org/10.1002/9781119350927.ch21
4. Merialdi, G., Dottori, M., Bonilauri, P., Luppi, A., Gozio, S., Pozzi, P., Spaggiari, B., Martelli, P. (2012). Survey of pleuritis and pulmonary lesions in pigs at abattoir with a focus on the extent of the condition and herd risk factors. Vet J. 193(1): 234 239. https://doi.org/10.1016/j.tvjl.2011.11.009 PMid:22182431
5. Fitzgerald, R.M., O’Shea, H., Manzanilla, E.G., Moriarty, J., McGlynn, H., Calderon Diaz J.A. (2020). Associations between animal and herd management factors, serological response to three respiratory pathogens and pluck lesions in finisher pigs on farrow-to-finish farms. Porcine Health Manag. 6(1): 34. https://doi.org/10.1186/s40813-020-00173-z PMid:33292673 PMCid:PMC7722331
6. Calsamiglia, M., Pijoan, C., Bosch, G.J. (1999). Profiling Mycoplasma hyopneumoniae in farms using serology and a nested PCR technique. Swine Health Prod. 7(6): 263-268.
7. Meyns, T., Van Steelant, J., Rolly, E., Dewulf, J., Haesebrouck, F., Maes, D. (2011). A crosssectional study of risk factors associated with pulmonary lesions in pigs at slaughter. Vet J. 187(3): 388392. https://doi.org/10.1016/j.tvjl.2009.12.027 PMid:20122861
8. Sibila, M., Bernal, R., Torrents, D., Riera, P., Llopart, D., Calsamiglia, M., Segales, J. (2008). Effect of sow vaccination against Mycoplasma hyopneumoniae on sow and piglet colonization and seroconversion, and pig lung lesions at slaughter. Vet Microbiol. 127(1-2): 165-170. https://doi.org/10.1016/j.vetmic.2007.07.027 PMid:17826008
9. Sibila, M., Calsamiglia, M., Vidal, D., Badiella, L., Aldaz, A., Jensen, J.C. (2004). Dynamics of Mycoplasma hyopneumoniae infection in 12 farms with different production systems. Can J Vet Res. 68(1): 12-18.
10. Madec, F., Kobish, M. (1982). Lesion assessment of the lungs of slaughter pigs at the slaughterhouse. [Bilanlesionnel des puiomons de porcscharcutiersal’abattoir]. J Rech Porc Fr. 14, 405 412. [In French]
11. Galdeano, J.V., Baraldi, T.G., Ferraz, M.E., de Souza Almeida, H.M., Mechler-Dreibi, M.L., Costa, W.M., Montassier, H.J., et al. (2019). Crosssectional study of seropositivity, lung lesions and associated risk factors of the main pathogens of Porcine Respiratory Diseases Complex (PRDC) in Goiás, Brazil. Porcine Health Manag. 5, 23. https://doi.org/10.1186/s40813-019-0130-0 PMid:31636919 PMCid:PMC6791015
12. Tassis, P.D., Tsakmakidis, I., Papatsiros, V.G., Koulialis, D., Nell, T., Brellou, G., Tzika, E.D. (2017). A randomized controlled study on the efficacy of a novel combination vaccine against enzootic pneumonia (Mycoplasma hyopneumoniae) and porcine Circovirus type 2 (PCV2) in the presence of strong maternally derived PCV2 immunity in pigs. BMC Vet Res. 13(1): 91. https://doi.org/10.1186/s12917-017-1014-7 PMid:28388953 PMCid:PMC5384188
13. Tzika, E.D., Tassis, P.D., Koulialis, D., Papatsiros, V.G., Nell, T., Brellou, G., Tsakmakidis, I. (2015). Field efficacy study of a novel ready-to-use vaccine against mycoplasma hyopneumoniae and porcine circovirus type 2 in a Greek farm. Porcine Health Manag, 1, 15. https://doi.org/10.1186/s40813-015-0006-x PMid:28405421 PMCid:PMC5382375
14. Petersen, A.C., Oneal, D.C., Seibel, J.R., Poel, K., Daum, C.L., Djordjevic, S.P., Minion, F.C. (2016). Cross reactivity among the swine mycoplasmas as identified by protein microarray. Vet Microbiol. 192, 204-212. https://doi.org/10.1016/j.vetmic.2016.07.023 PMid:27527784
15. Giacomini, E., Ferrari, N., Pitozzi, A., Remistani, M., Giardiello, D., Maes, D., Alborali, G.L. (2016). Dynamics of Mycoplasma hyopneumoniae seroconversion and infection in pigs in the three main production systems. Vet res Commun. 40(2): 81-88. https://doi.org/10.1007/s11259-016-9657-6 PMid:27142053
16. Hillen, S., von Berg, S., Kohler, K., Reinacher, M., Willems, H., Reiner, G. (2014). Occurrence and severity of lung lesions in slaughter pigs vaccinated against Mycoplasma hyopneumoniae with different strategies. Prev Vet Med. 113(4): 580-588. https://doi.org/10.1016/j.prevetmed.2013.12.012 PMid:24485705
17. Ostanello, F., Dottori, M., Gusmara, C., Leotti, G., Sala, V. (2007). Pneumonia disease assessment using a slaughterhouse lung‐scoring method. J Vet Med A Physiol Pathol Clin Med. 54(2): 70-75. https://doi.org/10.1111/j.1439-0442.2007.00920.x PMid:17305969
18. Andreasen, M., Mousing, J., Thomsen, L.K. (2001). No simple association between time elapsed from seroconversion until slaughter and the extent of lung lesions in Danish swine. Prev Vet Med. 52(2): 147-161. https://doi.org/10.1016/S0167-5877(01)00242-2 PMid:11679172
19. Stärk, K.D. (2000). Epidemiological investigation of the influence of environmental risk factors on respiratory diseases in swine-a literature review. Vet J. 159(1): 37-56.  https://doi.org/10.1053/tvjl.1999.0421 PMid:10640410 
20. Vicca, J., Maes, D., Thermote, L., Peeters, J., Haesebrouck, F., de Kruif, A. (2002). Patterns of Mycoplasma hyopneumoniae infections in Belgian farrow-to-finish pig herds with diverging diseasecourse. J Vet Med B Infect Dis Vet Public Helth. 49(7): 349-353. https://doi.org/10.1046/j.1439-0450.2002.00579.x PMid:12420871
21. Michiels, A., Vranckx, K., Piepers, S., Del Pozo Sacristan, R., Arsenakis, I., Boyen, F., Haesebrouck, F., Maes, D. (2017). Impact of diversity of Mycoplasma hyopneumoniae strains on lung lesions in slaughter pigs. Vet Res. 48, 2. https://doi.org/10.1186/s13567-016-0408-z PMid:28095890 PMCid:PMC5240301
22. Villarreal, I., Maes, D., Meyns, T., Gebruers, F., Calus, D., Pasmans, F., Haesebrouck, F. (2009). Infection with a low virulent mycoplasma hyopneumoniae isolate does not protect piglets against subsequent infection with a highly virulent M. hyopneumoniae isolate. Vaccine 27(12): 1875-1879. https://doi.org/10.1016/j.vaccine.2008.12.005 PMid:19114074

Copyright

© 2023 Angjelovski B. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Conflict of Interest Statement

The authors declared that they have no potential lict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 46, Issue 2, Pages 129-135, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2023-0018

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.