Short Communications
Immunohistochemical diagnostic characteristics of parvovirus infection in dogs
Ivica Gjurovski * ,
Spiro Bozinovski ,
Emilija Murdzevska ,
Todor Novakov ,
Mehmet Fatih Bozkurt ,
Trpe Ristoski

Mac Vet Rev 2023; 46 (2): 193 - 198

10.2478/macvetrev-2023-0024

Received: 10 February 2023

Received in revised form: 21 June 2023

Accepted: 10 July 2023

Available Online First: 24 July 2023

Published on: 15 October 2023

Correspondence: Ivica Gjurovski, igjurovski@fvm.ukim.edu.mk
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Abstract

Thе current study goal was to compare the results from the histopathological and immunohistochemical findings in dogs that have previously been clinically diagnosed with parvovirus infection. The Canine parvovirus enteritis (PVE) is a highly contagious disease mostly affecting dogs below six months of age. The canine parvovirus (CPV) belongs to the parvoviridae family within the feline parvovirus sub group of the genus Parvovirus. This investigation was performed on twenty dogs 2 to 12 months old with previous clinical diagnosis for PVE, and it included necropsy, histopathology, and immunohistochemistry. The necropsy findings included severe hemorrhagic enteritis and enlarged mesenteric lymph nodes as most frequent and prominent changes. The histopathological changes were also most significant in these organs in the form of villus atrophy and lymphoid depletion, respectively. These areas revealed the highest expression of CPV antigen. The histopathological and immunohistochemical methods provide strong base for a reliable CPV diagnosis.

Keywords: diagnosis, necropsy, histopathology, immunohistochemistry, canine parvovirus

References

1. Dogonyaro, B.B. (2010). Molecular characterization of canine parvovirus strains from domestic dogs in South Africa and Nigeria. Doctoral dissertation, University of Pretoria.
2. Appel, M.J., Scott, F.W., Carmichael, L.E. (1979). Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet Rec. 105(8): 156-159. https://doi.org/10.1136/vr.105.8.156 PMid:516347
3. Siegl, G., Bates, R.C., Berns, K.I., Carter, B.J., Kelly, D.C., Kurstak, E., Tattersall, P. (1985). Characteristics and taxonomy of Parvoviridae. Intervirology 23(2): 61-73. https://doi.org/10.1159/000149587 PMid:3980186
4. Gombač, M., Švara, T., Tadić, M., Pogačnik, M. (2008). Retrospective study of canine parvovirosis in Slovenia. Slov Vet Res. 45(2): 73-78.
5. Patterson, E.V., Reese, M.J., Tucker, S.J., Dubovi, E.J., Crawford, P.C., Levy, J.K. (2007). Effect of vaccination on parvovirus antigen testing in kittens. J Am Vet Med Assoc. 230(3): 359-363. https://doi.org/10.2460/javma.230.3.359 PMid:17269866
6. Pollock, R.V., Carmichael, L.E. (1982). Maternally derived immunity to canine parvovirus infection: transfer, decline, and interference with vaccination. J Am Vet Med Assoc. 180(1): 37-42.
7. Decaro, N., Elia, G., Martella, V., Campolo, M., Desario, C., Camero, M., Cirone, F., et al. (2006). Characterisation of the canine parvovirus type 2 variants using minor groove binder probe technology. J Virol Methods. 133(1): 92-99. https://doi.org/10.1016/j.jviromet.2005.10.026 PMid:16313976
8. Kahn, C.M., Line, S., Aiello, S.E. (2005). The Merck veterinary manual. Merck & Co. Inc., Whitehouse Station, NJ.
9. Hoskins, D.J. (1998). Canine viral enteritis. In: Greene, C.E. (Ed.), Infectious diseases of the dogs and cats. 2nd ed. (pp. 40-48). Philadelphia: W.B. Saunders Co.
10. Mosallanejad, B., Ghorbanpoor, N.M., Avizeh, R.R.A. (2008). Prevalence of canine parvovirus (CPV) in diarrheic dogs referred to veterinary hospital in Ahvaz. Arch Razi Inst. 63(2): 41-46.
11. Yilmaz, Z., Pratelli, A., Torun, S. (2005). Distribution of antigen types of canine parvovirus type 2 in dogs with hemorrhagic enteritis in Turkey. Turkish J Vet Anim Sci. 29(4): 1073-1076.
12. Ettinger, S.J., Feldman, E.C. (1995). Textbook of veterinary internal medicine. 4th. Ed. Section VIII: The Respiratory System. Philadelphia: W.B. Saunders.
13. Cooper, B.J., Carmichael, L.E., Appel, M.J., Greisen, H. (1979). Canine viral enteritis. II. Morphologic lesions in naturally occurring parvovirus infection. Cornell Vet. 69(3): 134-144.
14. Hayes, M.A., Russel, R.G., Mueller, R.W., Lewis, R.J. (1979). Myocarditis in young dogs associated with a parvovirus-like agent. Can Vet J. 20(5): 126.
15.Robinson, W.F., Huxtable, C.R., Pass, D.A. (1980). Canine parvoviral myocarditis: a morphologic description of the natural disease. Vet Pathol. 17(3): 282-293. https://doi.org/10.1177/030098588001700302 PMid:7368523
16. Bergmann, V., Baumann, G., Fuchs, H.W. (1980). Experiences in the postmortem diagnosis of parvovirus enteritis in the dogs. [Zur postmortalen Diagnostik der Parvovirus-Enteritis des Hundes]. Arch Exp Veterinarmed. 37(1): 127-133. [in German]
17. Breuer, W., Stahr, K., Majzoub, M., Hermanns, W. (1998). Bone-marrow changes in infectious diseases and lymphohaemopoietic neoplasias in dogs and cats-a retrospective study. J Comp Pathol. 119(1): 57-66. https://doi.org/10.1016/S0021-9975(98)80071-6 PMid:9717127
18. Vural, S.A., Alcigir, G. (2011). Histopathological and immunohistological findings in canine parvoviral infection: diagnosis application. Revue Méd Vét. 162(2): 59-64.
19. Maxson, T.R., Meurs, K.M., Lehmkuhl, L.B., Magnon, A.L., Weisbrode, S.E., Atkins, C.E. (2001). Polymerase chain reaction analysis for viruses in paraffin-embedded myocardium from dogs with dilated cardiomyopathy or myocarditis. Am J Vet Res. 62(1): 130-135. https://doi.org/10.2460/ajvr.2001.62.130 PMid:11197551
20. Mulvey, J.J., Bech-Nielsen, S., Haskins, M.E., Taylor, H.W., Eugster, A.K. (1980). Myocarditis induced by parvoviral infection in weanling pups in the United States. J Am Vet Med Assoc. 177(8): 695-698.
21. Desario, C., Decaro, N., Campolo, M., Cavalli, A., Cirone, F., Elia, G., Martella, V., et al. (2005). Canine parvovirus infection: which diagnostic test for virus? J Virol Methods. 126(1-2): 179-185. https://doi.org/10.1016/j.jviromet.2005.02.006 PMid:15847935
22. Waldvogel, A.S., Hassam, S., Stoerckle, N., Weilenmann, R., Tratschin, J.D., Siegl, G., Pospischil, A. (1992). Specific diagnosis of parvovirus enteritis in dogs and cats by in situ hybridization. J Comp Pathol. 107(2): 141-146. https://doi.org/10.1016/0021-9975(92)90031-O PMid:1333495
23. Gjurovski, I., Angelovski, B., Dovenski, T., Mitrov, D., Ristoski, T. (2015). Diagnostic characteristics of circovirus infection in pigs. Mac Vet Rev. 38(1): 73-78. https://doi.org/10.14432/j.macvetrev.2014.11.033 
24. Vural, S.A., Alcigir, G. (2011). Histopathological and immunohistological findings in canine parvoviral infection: diagnosis application. Revue Méd Vét. 162(2): 59-64.
25. Gjurovski, I., Dovenska, M., Kunovska, S.K., Milosevski, J., Trajkov, V.L., Ristoski, T. (2019). Mammary adenocarcinoma with widespread metastasis in a lion. Mac Vet Rev. 42(2): 195-199. https://doi.org/10.2478/macvetrev-2019-0019 
26. Azetaka, M., Hirasawa, T., Konishi, S., Ogata, M. (1981). Studies on canine parvovirus isolation, experimental infection and serologic survey. Nihon Juigaku Zasshi. 43(2): 243-255. https://doi.org/10.1292/jvms1939.43.243 PMid:7289337
27. Danner, K., Oldenburg, U., Weber, S., Arens, M., Krauss, H. (1982). Current epizootiological situation in virus-related canine enteritis. [Derzeitige epizootiologische Situation bei virusbedingten Enteritiden des Hundes]. Praktische Tierarzt. 63, 512 - 518. [in German]
28. Jakov, N., Nenad, M., Andrea, R., Dejan, K., Dragan, M., Aleksandra, K., Marina, R., et al. (2021). Genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in Serbia. Acta Veterinaria 71(1): 32-46. https://doi.org/10.2478/acve-2021-0003 
29. Kubašová, I., Štempelová, L., Maďari, A., Bujňáková, D., Micenková, L., Strompfová, V. (2022). Application of canine-derived DSM 32820 in dogs with acute idiopathic diarrhoea. Acta Veterinaria 72(2): 167-183. https://doi.org/10.2478/acve-2022-0014 
30. Evermann, J.F., Abbott, J.R., Han, S. (2005). Canine coronavirus-associated puppy mortality without evidence of concurrent canine parvovirus infection. J Vet Diagn. 17(6): 610-614. https://doi.org/10.1177/104063870501700618 PMid:16475526
31. Url, A., Schmidt, P. (2005). Do canine parvoviruses affect canine neurons? An immunohistochemical study. Res Vet Sci. 79(1): 57-59. https://doi.org/10.1016/j.rvsc.2004.11.003 PMid:15894025
32. Berkin, S., Milli, U., Urman, H.K. (1981). Canine parvoviral enteritis in Turkey. Vet J Ank Uni. 28, 36-49.

Copyright

© 2023 Gjurovski I. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Conflict of Interest Statement

The authors declared that they have no potential lict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 46, Issue 2, Pages 193-198, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2023-0024

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.