Short Communications
The effects of Bifidobacterium animalis subsp. lactis and vitamin D on immunological response following vaccination in puppies
Gökhan Saridag ,
Songul Erdogan ,
Tahir Ozalp ,
Kerem Ural ,
Hasan Erdogan *

Mac Vet Rev 2023; 46 (2): 199 - 206


Received: 27 March 2023

Received in revised form: 01 July 2023

Accepted: 09 August 2023

Available Online First: 15 September 2023

Published on: 15 October 2023

Correspondence: Hasan Erdogan,


This study aimed to determine the effects of oral application of Bifidobacterium animalis subsp. lactis and vitamin D on the immune response after vaccination in puppies. Crossbred dogs at the age of 55–65 days (n=21) of both sexes were grouped according to the application as follows: Group 1 - commercial polyvalent vaccine only (Vanguard 5L4, Zoetis); Group 2 - polyvalent vaccine and vitamin D; and Group 3 - polyvalent vaccine, B. animalis subsp. lactis, and vitamin D. The antibody titers, measured 3 weeks after the vaccination and after the 1st booster, demonstrated sufficient protective levels against the canine distemper virus but without significant difference between the groups when compare both values. No significant differences either, were observed in antibody titers against canine parvovirus after the initial vaccination. However, after the 1st booster, Group 2 samples showed significantly higher antibody titer value compared to the other groups (p<0.05). In conclusion, the dogs included in this study had an adequate antibody response to canine parvovirus and distemper viruses following the vaccination and 1st booster in all three groups of animals, and significant increasing of protective antibody titers against canine parvovirus virus the after the 1st booster in the group where B. animalis subsp. lactis was included.

Keywords: antibody response, Bifidobacterium animalis subsp. lactis, canine parvovirus, vaccination, vitamin D


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© 2023 Ergodan H. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Conflict of Interest Statement

The authors declared that they have no potential lict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 46, Issue 2, Pages 199-206, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2023-0025