Macedonian Veterinary Review

Original Scientific Article

Distribution and genotyping of infectious hematopoietic necrosis virus in farmed rainbow trout and autochthonous salmonids in North Macedonia

Aleksandar Traјchovski *

Igor Djadjovski

Kiril Krstevski

Zagorka Popova-Hristovska

Martin Nikolovski

Ljubica Rashikj

Dimitar Bozinovski

Angela Grujovska ,

Niccolò Vendramin

Argelia Cuenca

Aleksandar Cvetkovikj

Mac Vet Rev 2024; 47 (1): 37 - 43

10.2478/macvetrev-2024-0013

Received: 18 December 2023

Received in revised form: 29 January 2024

Accepted: 06 February 2024

Available Online First: 13 February 2024

Published on: 15 March 2024

Correspondence: Aleksandar Traјchovski, aleksandar.trajchovski@fvm.ukim.edu.mk

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Abstract

Infectious hematopoietic necrosis (IHN) is a common disease in the intensive production of salmonids. The IHN virus (IHNV) was isolated for the first time in North Macedonia in 2018. This study aimed to investigate the distribution and genotype of IHN in farmed rainbow trout and autochthonous salmonid fish in North Macedonia following the first detection. The samples were collected from 47 trout farms. Trout fry with or without clinical signs of IHN were selected as individual samples. Kidney, spleen, and heart were taken from each fish during the dissection. Three pooled samples were collected from each farm. A total of 141 pooled samples were collected: Rainbow trout (Oncorhynchus mykiss) n=127, Macedonian trout (Salmo macedonicus) n=11, and Ohrid trout (Salmo letnica) n=3. The virus was detected in 43 samples (30.50%): rainbow trout (n=40), Macedonian trout (n=2), and Ohrid trout (n=1). There were 18 (38.30%) positive fish farms. The MAKIHNV1 isolate from 2018 (MN641902) and the newly isolated virus shared a similarity of >99 and were placed in clade E-1 of European genogroup E. The IHN has spread throughout the country and is also present in the autochthonous salmonids.

Keywords: Novirhabdovirus, cold-water aquaculture, phylogeny

References

1. Dixon, P., Paley, R., Alegria-Moran, R., Oidtmann, B. (2016). Epidemiological characteristics of infectious hematopoietic necrosis virus (IHNV): a review. Vet Res. 47(1): 63. https://doi.org/10.1186/s13567-016-0341-1 PMid:27287024 PMCid:PMC4902920

2. Verjan, N., Ooi, E.L., Nochi, T., Kondo, H., Hirono, I., Aoki, T., Kiyono, H., Yuki, Y. (2008). A soluble nonglycosylated recombinant infectious hematopoietic necrosis virus (IHNV) G-protein induces IFNs in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol. 25(1-2): 170-180. https://doi.org/10.1016/j.fsi.2008.04.004 PMid:18499475

3. Yong, C.Y., Ong, H.K., Tang, H.C., Yeap, S.K., Omar, A.R., Ho, K.L., Tan, W.S. (2019). Infectious hematopoietic necrosis virus: advances in diagnosis and vaccine development. PeerJ. 7, e7151. https://doi.org/10.7717/peerj.7151 PMid:31341728 PMCid:PMC6640626

4. Rucker, R.R., Whipple, W.J., Parvin, J.R., Evans, C.A., Service, W., Day, A.M. (1953). A contagious disease of sockeye salmon possibly of virus origin. US Fish Wildlife Service Fisheries Bulletin. 54(1): 35-46.
5. Watson, S.W., Guenther, R.W., Rucker, R.R. (1954). Virus disease of sockeye salmon, interim report. United States Fish Wildlife Special Scientific Report on Fisheries. 138, 1-36.
6. Enzmann, P.J., Castric, J., Bovo, G., Thiery, R., Fichtner, D., Schütze, H., Wahli, T. (2010). Evolution of infectious hematopoietic necrosis virus (IHNV), a fish rhabdovirus, in Europe over 20 years: implications for control. Dis Aquat Organ. 89(1): 9-15. https://doi.org/10.3354/dao02182 PMid:20391908
7. Grilc, F.A, Jenčič, V., Mankoč, S., Maganja, D.B., Hostnik, P. (2011). Genotyping of Slovenian infectious hematopoietic necrosis virus isolates based on the “mid-G” region sequences of the glycoprotein gene. Bull Eur Assoc Fish Pathol. 31(2): 47-57.
8. Vardić, I., Kapetanović, D., Teskeredžić, Z., Teskeredžić, E. (2007). First record of infectious haematopoietic necrosis virus in rainbow trout fry in Croatia. Acta Vet Brno. 76(1): 65-70. https://doi.org/10.2754/avb200776010065
9. Rexhepi, A., Bërxholi, K., Scheinert, P., Hamidi, A., Sherifi, K. (2011). Study of viral diseases in some freshwater fish in the Republic of Kosovo. Veti Arh. 81(3): 405-413.
10. Cvetkovikj, A., Radosavljevic, V., Cuenca, A., Strojmanovska, B., Maksimovic-Zoric, J., Cvetkovikj, I., Olesen, N.J. (2020). First detection of infectious haematopoietic necrosis virus in farmed rainbow trout in North Macedonia. Dis Aquat Organ. 140, 219-225. https://doi.org/10.3354/dao03507 PMid:32880379
11. Enzmann, P.J., Kurath, G., Fichtner, D., Bergmann, S.M. (2005). Infectious hematopoietic necrosis virus: monophyletic origin of European isolates from North American genogroup M. Dis Aquat Organ. 66(3): 187-195. https://doi.org/10.3354/dao066187 PMid:16261933
12. Vardić, I., Kapetanović, D., Teskeredžić, Z., Teskeredžić, E. (2007). Genotyping of isolated viruses from rainbow trout (Oncorhynchus mykiss) in Croatia. Ribarstvo 65(3): 87-97. https://doi.org/10.2754/avb200776010065
13. Purcell, M.K., Thompson, R.L., Garver, K.A., Hawley, L.M., Batts, W.N., Sprague, L., Sampson, C., Winton, J.R. (2013). Universal reverse-transcriptase real-time PCR for infectious hematopoietic necrosis virus (IHNV). Dis Aquat Organ. 106(2): 103-115. https://doi.org/10.3354/dao02644 PMid:24113244
14. Ahmadivand, S., Soltani, M., Mardani, K., Shokrpoor, S., Hassanzadeh, R., Ahmadpoor, M., Rahmati- Holasoo, H., Meshkini, S. (2017). Infectious hematopoietic necrosis virus (IHNV) outbreak in farmed rainbow trout in Iran: viral isolation, pathological findings, molecular confirmation, and genetic analysis. Virus Res. 229, 17-23. https://doi.org/10.1016/j.virusres.2016.12.013 PMid:28012997
15. Barroso, R.M., Wheeler, P.A., Lapatra, S.E., Drewer, R.E., Thoragaard, G.H. (2008). QTL for IHNV resistance and growth identified in a rainbow trout (Oncorhynchus mykiss) X Yellowstone cutthroat (Oncorhynchus clarki bouvieri) trout cross. Aquaculture. 277(3-4): 156-163. https://doi.org/10.1016/j.aquaculture.2008.03.001
16. Balmer, B.F., Powers, R.L., Zhang, T.H., Lee, J., Vigant, F., Lee, B., Jung, M.E., Purcell, M.K., Snekvik, K., Aguilar, H.C. (2017). Inhibition of an aquatic rhabdovirus demonstrates promise of a broad-spectrum antiviral for use in aquaculture. J Virol. 91(4): e02181-16. https://doi.org/10.1128/JVI.02181-16 PMid:27903801 PMCid:PMC5286899
17. Adams, A. (2019). Progress, challenges and opportunities in fish vaccine development. Fish Shellfish Immunol. 90, 210-214. https://doi.org/10.1016/j.fsi.2019.04.066 PMid:31039441

Copyright

© 2024 Trajchovski A. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Conflict of Interest Statement

The authors declared that they have no potential lict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 47, Issue 1, Pages 37-43, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2024-0013