Macedonian Veterinary Review

Original Scientific Article

Immunohistochemical detection of CD3 and MAC387 antibodies in the mesenteric lymph nodes and the small intestine of 20 dogs that died of canine parvoviral enteritis

Todor Novakov

Ivica Gjurovski *

Spiro Bozinovski ,

Aleksandar Janevski

Nikola Adamov

Trpe Ristoski

Mac Vet Rev 2024; 47 (1): 59 - 64

10.2478/macvetrev-2024-0014

Received: 09 January 2024

Received in revised form: 07 February 2024

Accepted: 13 February 2024

Available Online First: 29 February 2024

Published on: 15 March 2024

Correspondence: Ivica Gjurovski, igjurovski@fvm.ukim.edu.mk

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Abstract

There is little data on the use of the immunohistochemical method in the observation of the T lymphocytes and macrophages distribution within the mesenteric lymph nodes in canine parvovirus (CPV) enteritis. The virus initially replicates in the systemic lymphoid tissue and causes highest changes in the small intestine. This current study aimed to demonstrate the CD3 and MAC387 antibodies detection and distribution in mesenteric lymph nodes and small intestines in dogs which had positive clinical, pathological, and histological findings of CPV. Twenty dogs that were clinically confirmed on CPV, had been autopsied following onset of death. Tissue samples (lymph nodes and small intestines) from each dog was processed for histology and immunohistochemistry utilizing hematoxylin-eosin and peroxidase/DAB (3,3′-diaminobenzidine) staining methods, respectively. The virus distribution was greatest in the tissue areas of the small intestine and the lymph nodes where the virus had made the most severe tissue damage. The distribution of macrophage was highest in the necrotic areas of the small intestine and the mesenteric lymph nodes. The amount of CD3 positive lymphocytes was greatly reduced in the mesenteric lymph nodes and the lymphoid tissue of the small intestine.

Keywords: immunohistochemistry, lymphocytes, macrophage, parvovirus

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Copyright

© 2024 Novakov T. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Conflict of Interest Statement

The authors declared that they have no potential lict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 47, Issue 1, Pages 59-64, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2024-0014