Original Scientific Article
External progesterone supplementation during the Ovsynch protocol reduces the incomplete luteolysis in dairy cows under heat stress
Boris Stojanov ,
Branko Atanasov * ,
Juraj Grizelj ,
Silvijo Vince ,
Ksenija Ilievska ,
Martin Nikolovski ,
Toni Dovenski ,
Marko Samardžija

Mac Vet Rev 2024; 47 (1): 45 - 50


Received: 26 December 2023

Received in revised form: 18 February 2024

Accepted: 24 February 2024

Available Online First: 27 February 2024

Published on: 15 March 2024

Correspondence: Branko Atanasov, batanasov@fvm.ukim.edu.mk


The present study aimed to determinate the effect of external progesterone (P4) supplementation on luteolysis in cows under heat stress. Forty-eight (n=48) dairy cows in the period from July September 2018 were part of and at day 35±3 postpartum scored for BSC, synchronized using PG-3-G + Ovsynch protocol and randomly allocated into two treatments: PRID group (n=27) treated with external P4 device between G1 and PGF and CON group (n=21) left without treatment. Collection of blood samples to assess P4 concentrations was done at Pre-PG, at G1, at PGF, at 72 h after PGF (at timed artificial insemination TAI) and at d 21 after TAI. The pregnancy diagnosis was done at d 21 and d 30 after TAI by ultrasound. The average temperature humidity index (THI) was 79.5±0.6. At G1, the P4 was significantly lower in the PRID group (1.84±0.99 ng/mL) in comparison to the CON group (2.97±1.82 ng/mL). In contrast, at PGF, there was a tendency (p=0.09) of increased P4 concentration in PRID group compared with the CON group (4.26±1.68 and 3.74±2.39 ng/mL), respectively. At TAI, more PRID cows (p=0.0001) had a lower P4 (0.06±0.03 ng/mL), in comparison to CON (1.28±2.41 ng/mL). At d 21 and d 30 after TAI, more PRID cows were predicted and diagnosed pregnant (16/27 or 59.25% and 13/27 or 48.14%) compared with the CON group (11/21 or 52.38% and 8/21 or 38.08%) respectively, but without any significant differences. Supplementation of the P4 during the Ovsynch protocol increases the P4 before TAI and reduces the incomplete luteolysis in heat stressed dairy cows.

Keywords: cows, heat stress, Ovsynch, luteolysis, PRID


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© 2024 Stojanov B. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Conflict of Interest Statement

The authors declared that they have no potential lict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 47, Issue 1, Pages 45-50, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2024-0015