Original Scientific Article
Rabies virus nucleoprotein: large-scale expression in prokaryotic system
Leandro Daniel Picotto ,
Carlos Javier Panei * ,
Marcelo Ricardo Pecoraro ,
Guillermo Hernán Sguazza

Mac Vet Rev 2024; 47 (2): 151 - 158

10.2478/macvetrev-2024-0026

Received: 05 April 2024

Received in revised form: 13 August 2024

Accepted: 15 August 2024

Available Online First: 03 September 2024

Published on: 15 October 2024

Correspondence: Carlos Javier Panei, javierpanei@fcv.unlp.edu.ar
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Abstract

Rabies is controlled by mass animal vaccination campaigns. Cats, dogs, and wild animals (e.g., bats) are large reservoirs of this virus and can pose a significant threat to the human health, especially in the developing countries. The nucleoprotein of the rabies virus is of great scientific interest since it has the potential to generate immunity in animals and can be used as for immunochemical diagnostics. The study aimed to test a large-scale expression of the rabies N protein in a prokaryotic system. The recombinant N protein was successfully expressed and purified. It was immunologically recognized by specific antibodies and was able to induce the production of specific antibodies in a mouse immunization assay. These encouraging results indicate that the recombinant N protein can be evaluated as an antigen for the development of a subunit vaccine or for a diagnostic assay.

Keywords: rabies virus, nucleoprotein, Escherichia coli, large-scale expression

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Copyright

© 2024 Picotto L.D. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Conflict of Interest Statement

The authors declared that they have no potential lict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 47, Issue 2, Pages 151-158, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2024-0026

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.