Original Scientific Article
Use of nano-collagen creams extracted from catfish skin in treatment of second-degree skin burns in rabbits
Ahmed K. Munahi ,
Abbas Ali H. Allban * ,
Rafid H. Farman ,
Amir I. Towfik

Mac Vet Rev 2025; 48 (2): i - xii

10.2478/macvetrev-2025-0021

Received: 22 June 2024

Received in revised form: 19 February 2025

Accepted: 13 March 2025

Available Online First: 16 April 2025

Published on: 15 October 2025

Correspondence: Abbas Ali H. Allban, abbas.ali@qu.edu.iq
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Abstract

Second-degree burns typically require a longer healing period and can develop hypertrophic scars. Collagen plays a crucial role in treating and healing skin wounds. This study aimed to investigate the effect of topically applied nano-collagen extracted from catfish skin on rabbit burn wounds by assessing the production of collagen and the development of fibroblasts in the healing wounds. The nano-collagen material was produced in two stages by using NaOH and alcohol solutions in the first, and acetic acid solution in the second phase. The viscous solution obtained in this process was adjusted to a specific pH, was centrifuged, and was mixed with Vaseline before being lyophilized. Thirty skin samples from burn wounds were obtained from blemish-free rabbits. Each rabbit was treated by inflicting a 50 mm2 burn wound on the back. They were divided into three subgroups: a control group (G1, n=10) without wound treatment, a group topically treated with nano-collagen cream on a daily basis for one week (G2, n=10) and a group treated by sewing catfish skin onto their wounds (G3, n=10). Measurements were taken daily during the first week to monitor any possible post-burn contractions. G2 had significantly the lowest wound diameter after 7 days of treatment (35.00±1.21 mm). It had significantly the lowest wound contraction (8.200±0.042 kg/cm2) on 21 days of the treatment and had higher observable vascularization and development of hair follicles in the wounds compared to the other groups. In conclusion, the topical application of catfish skin nano-collagen cream significantly reduces second-degree burn wounds in rabbits and improves the healing process.

Keywords: nano-collagen, catfish skin, burn wound, skin, rabbits

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Copyright

© 2025 Munahi A.K. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors declare that they have no financial or non-financial conflict of interest regarding authorship and publication of this article .

Citation Information

Macedonian Veterinary Review. Volume 48, Issue 2, Pages i-xii, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI:  https://doi.org/10.2478/macvetrev-2025-0021

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.