Original Scientific Article
Comparative study of 3D reconstruction techniques on rhinoceros iguana skull by utilizing computer tomography scans
José Raduan Jaber * ,
Mario Encinoso ,
Alejandro Morales-Espino ,
Francisco Suárez-Cabrera

Mac Vet Rev 2025; 48 (2): i - xii

10.2478/macvetrev-2025-0030

Received: 11 August 2024

Received in revised form: 21 July 2025

Accepted: 07 August 2025

Available Online First: 02 October 2025

Published on: 15 October 2025

Correspondence: José Raduan Jaber, joseraduan.jaber@ulpgc.es
PDF HTML

Abstract

The rhinoceros iguana is an endangered species native to the Caribbean, currently listed on the International Union for Conservation of Nature (IUCN) Red List. While most available studies focus on its ecology and conservation, there is a lack of detailed anatomical descriptions, especially of the skull, which limits clinical, educational, and research applications in veterinary medicine. Computed tomography (CT) and three-dimensional (3D) reconstructions, provides a non-invasive and precise method to identify and annotate anatomical structures in reptiles. This study aimed to evaluate and illustrate the anatomical fidelity and utility of different CT-based reconstruction techniques, using Cyclura cornuta cornuta as a representative model. We applied three post-processing methods-Volume Rendering (VR3D), Maximum Intensity Projection (MIP), and Multiplanar Reformatting with Volume Rendering (MPR-VR)-to two female adult specimens. Our results demonstrate that each technique offers unique advantages in visualizing cranial features, and that combining them improves anatomical interpretation. This work provides a practical imaging reference for reptile skull anatomy and supports future applications in clinical diagnostics, comparative morphology, and exotic animal education.

Keywords: rhinoceros iguana, skull anatomy, computer tomography, 3D reconstruction


References

  1. Arantes, R.C., Soares, M. de J.V., Lima, A.K.F., Carneiro E Silva, F.O., de Oliveira, A. das G.H. (2016). The skull bones of the Iguana iguana. Biosci J. 32(1): 219-225. https://doi.org/10.14393/BJ-v32n1a2016-30045 
  2. Holmes, S.P., Divers, S.J. (2019). Radiography- general principles. In: S.J. Divers, S.J. Stahl (Eds.), Mader’s reptile and amphibian medicine and surgery. 3th ed. (pp. 486-490). W.B. Saunders Company https://doi.org/10.1016/B978-0-323-48253-0.00053-2 
  3. Banzato, T., Selleri, P., Veladiano, I.A., Zotti, A. (2013). Comparative evaluation of the cadaveric and computed tomographic features of the coelomic cavity in the Green iguana (Iguana iguana), Black and White tegu (Tupinambis merianae) and Bearded dragon (Pogona vitticeps). Anat Histol Embryol. 42(6): 453-460. https://doi.org/10.1186/1746-6148-8-53 PMid:22578088 PMCid:PMC3439268
  4. Banzato, T., Selleri, P., Veladiano, I.A., Martin, A., Zanetti, E., Zotti, A. (2012). Comparative evaluation of the cadaveric, radiographic and computed tomographic anatomy of the heads of Green iguana (Iguana iguana), Common tegu (Tupinambis merianae) and Bearded dragon (Pogona vitticeps). BMC Vet Res. 8, 53. https://doi.org/10.1186/1746-6148-8-53 PMid:22578088 PMCid:PMC3439268
  5. Pasachnik, S.A., Colosimo, G., Carreras-De León, R., Gerber, G. (2020). Genetic structure of Rhinoceros rock iguanas, Cyclura cornuta, in the Dominican Republic, with insights into the impact of captive facilities and the taxonomic status of Cyclura on Mona Island. Conserv Genet. 21(5): 837-851. https://doi.org/10.1007/s10592-020-01290-6
  6. Reynolds, R.G., Miller, A.H., Pasachnik, S.A., Knapp, C.R., Welch, M.E., Colosimo, G., Gerber, G.P., et al. (2022). Phylogenomics and historical biogeography of West Indian Rock Iguanas (Genus Cyclura). Mol Phylogenet Evol. 174, 107548. https://doi.org/10.1016/j.ympev.2022.107548 PMid:35690377
  7. Grant, T.D., Hudson, R.D. (2015). West Indian iguana Cyclura spp. reintroduction and recovery programmes: Zoo support and involvement. Int Zoo Yearb. 49(1): 49-55. https://doi.org/10.1111/izy.12078 
  8. Mehler, S.J., Rosenstein, D.S., Patterson, J.S. (2002). Imaging diagnosis follicular torsion in a green iguana (Iguana iguana) with involvement of the left adrenal gland. Vet Radiol Ultrasound. 43(4): 343-345. https://doi.org/10.1111/j.1740-8261.2002.tb01015.x PMid:12174997
  9. Ferguson, G.W., Gehrmann, W.H., Bradley, K.A., Lawrence, B., Hartdegen, R., Storms, T., Chen, T.C., Holick, M.F. (2015). Summer and winter seasonal changes in vitamin D Status of captive Rhinoceros iguanas (Cyclura cornuta). J Herpetol Med Surg. 25(3-4): 128-136. https://doi.org/10.5818/1529-9651-25.3.128
  10. González Rodríguez, E., Encinoso Quintana, M., Morales Bordon, D., Garcés, J.G., Artiles Nuez, H., Jaber, J.R. (2023). Anatomical description of Rhinoceros iguana (Cyclura cornuta cornuta) head by computed tomography, magnetic resonance imaging and gross-sections. Animals. 13(6): 955. https://doi.org/10.3390/ani13060955 PMid:36978497 PMCid:PMC10044561
  11. Jaber, J.R., González-Rodríguez, E., Arencibia, A., Deniz, S., Carrascosa, C., Encinoso, M. (2023). Anatomical description of Loggerhead turtle (Caretta caretta) and Green iguana (Iguana iguana) skull by three-dimensional computed tomography reconstruction and maximum intensity projection images. Animals (Basel). 13(4): 621. https://doi.org/10.3390/ani13040621 PMid:36830407 PMCid:PMC9951731
  12. Banzato, T., Hellebuyck, T., Van Caelenberg, A., Saunders, J.H., Zotti, A.A. (2013). Review of diagnostic imaging of snakes and lizards. Vet Rec. 173(2): 43-49. https://doi.org/10.1136/vr.101450 PMid:23857534
  13. Broeckhoven, C., Du Plessis, A. (2018). X-ray microtomography in herpetological research: a review. Amphib Reptil. 39(4): 377-401. https://doi.org/10.1163/15685381-20181102
  14. Pérez, S., Encinoso, M., Morales, M., Arencibia, A., Suárez-Bonnet, A., González-Rodríguez, E., Jaber, J.R. (2021). Comparative evaluation of the Komodo dragon (Varanus komodoensis) and the Green iguana (Iguana iguana) skull by three-dimensional computed tomographic reconstruction. Slov Vet Res. 58(3): 111-116. https://doi.org/10.26873/SVR-1330-2021
  15. Fishman, E.K., Ney, D.R., Heath, D.G., Corl, F.M., Horton, K.M., Johnson, P.T. (2006). Volume rendering versus maximum intensity projection in CT angiography: what works best, when, and why. Radiographics. 26(3): 905-922. https://doi.org/10.1148/rg.263055186 PMid:16702462 
  16. Calhoun, P.S., Kuszyk, B.S., Heath, D.G., Carley, J.C., Fishman, E.K. (1999). Three-dimensional volume rendering of spiral CT data: theory and method. Radiographics. 19(3): 745-764. https://doi.org/10.1148/radiographics.19.3.g99ma14745 PMid:10336201
  17. Evans, S.E. (2008). The skull of lizards and Tuatara. In: C. Gans, A.S. Gaunt, K. Adler (eds.), Biology of the reptilia, volume 20 (p. 1-344.) Morphology H: the skull of Lepidosauria. Society for the Study of Amphibians and Reptiles, Ithaca, New York
  18. Paparella, I., Caldwell, M.W. (2022). Cranial anatomy of         the       Galápagos            marine iguana Amblyrhynchus cristatus (Squamata: Iguanidae). Anat Rec (Hoboken). 305(7): 1739-1786. https://doi.org/10.1002/ar.24797 PMid:34652885
  19. Jaber, J.R., Carrascosa, C., Arencibia, A., Corbera J.A., Ramirez, A.S., Melian, C. (2018). 3-D computed tomography reconstruction: another tool to teach anatomy in the veterinary colleges. Iran J Vet Res. 19(1): 1-2.


Copyright

© 2025 Jaber J.R. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors declare that they have no financial or non-financial conflict of interest regarding authorship and publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 48, Issue 2, Pages i-xii, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: https://doi.org/10.2478/macvetrev-2025-0030