Macedonian Veterinary Review

Original Scientific Article

Comparative study of glucose transporters GLUT-2 and GLUT-5 in ostriches gastrointestinal tract

Piret Hussar * ,

Martin Kärner ,

Tõnu Järveots ,

Lazo Pendovski ,

Ilmars Duritis ,

Florina Popovska-Percinic

Mac Vet Rev 2016; 39 (2): 225 - 231

10.1515/macvetrev-2016-0089

Received: 22 January 2016

Received in revised form: 25 April 2016

Accepted: 30 June 2016

Available Online First: 25 July 2016

Published on: 15 October 2016

Correspondence: Piret Hussar, piretut@gmail.com

PDF HTML

Abstract

The knowledge about transport of sugars in animals and birds gastrointestinal tract is very important for science as carbohydrates are the main energy source of food. Since until now there is few information about the localization of glucose transporters - integral membrane proteins that mediate the transport of glucose and related substances across the cellular membranes - in birds gastrointestinal tract, the aim of the present study was to localize glucose transporters-2 and -5 (GLUT-2 and -5) in three parts of the ostriches gastrointestinal tract – proventriculus, duodenum and ileum - comparatively in ostrich chicken in their early ontogenesis period. Material from the superficial gland zone of the proventriculus, duodenum and terminal zone of the ileum were collected from eight female ostriches (Struthio camelus var. Domesticus): two chickens after hatching, three 7 and three 30-days old ostriches. The material was fixed with 10% formalin, embedded into paraffin, slices 7 μm thick were cut followed by immunohistochemical staining with polyclonal primary antibodies Rabbit anti-GLUT-2 and Rabbit anti-GLUT-5, carried out according to the manufacturers guidelines (IHC kit, Abcam, UK). The results showed that the staining for both antibodies was weaker in all parts of the gastrointestinal tract of ostriches after hatching compared to 7 and 30 days old ostriches showing that the gastrointestinal tract of ostriches immediately after hatching is not entirely capable of transportation of carbohydrates. The results of our study may indicate the possibility of a close relationship between feeding and the ability to transport sugars in the gastrointestinal tract.

Keywords: ostriches, gastrointestinal tract, GLUT-2, GLUT-5

References

1. Davis K, Nowhere to hide 1993; 3: Poultry Press; 4-

2. Duritis I, Morphofunctional characterization of the stomach and small intestine of the ostrich (Struthio camelus var. domesticus) from day 38 of embryonic development to the age of 60 daysDoctoral dissertation (in Latvian) 2011; Jelgava, Latvia: Latvia University of Agriculture;

3. Cloete S.W.P, Lambrechts H, Punt K, Brand Z, Factors related to high levels of ostrich chick mortality from hatching to 90 days of age in an intensive rearing systemJournal of the South African Veterinary Association 2001; 72: 4197-202. http://dx.doi.org/10.4102/jsava.v72i4.652 PMid: 12219914

4. Fritz J, Hummel J, Kienzle E, Gizzard vs. teeth, it’s a tie: food-processing efficiency in herbivorous birds and mammals and implications for dinosaur feeding strategiesPaleobiology 2011; 37: 577-586. http://dx.doi.org/10.1666/10031.1

5. Bezuidenhout A.J, Van Aswegen G, A light microscopic and immunohistochemical study of the gastrointestinal tract of the ostrich (Struthio Camelus L.)Onderstepoort J. Vet. Res 2011; 57: 37-48.

6. Cho P, Brown R, Anderson M, Comparative gross anatomy of ratitesZoo Biology 1984; 3: 133-144. http://dx.doi.org/10.1002/zoo.1430030205

7. Cooper R.G, Mahroze K.M, Anatomy and physiology of the gastro-intestinal tract and growth curves of the ostrich (Struthio camelus)Animal Science Journal 2004; 75: 491-498. http://dx.doi.org/10.1111/j.1740-0929.2004.00218.x

8. Illanes J, Fertilio B, Chamblas M, Histologic description of the different segments from the ostrich digestive system (Struthio camelus var. domesticus)Int J Morphol 2006; 24: 2205-214. http://dx.doi.org/10.4067/S0717-95022006000300015

9. Wang J.X, Peng K.M, Du A.N, Histological study on the digestive ducts of African ostrich chicksChin J Zool 2007; 42: 3131-135.

10. Duritis I, Mugurevics A, Mancevica L, The distribution of gastrin, somatostatin and glucagon immunoreactive (IR) cells in ostrich stomach during the pre- and post-hatching periodAnatomia Histologia Embryologia 2013; 42: 5362-368. http://dx.doi.org/10.1111/ahe.12023 PMid: 23293918

11. Hussar P, Järveots T, Rotmistrova A, Immunohistochemical localization of glucose transporters in ostriches gastrointestinal tractWulfenia 2014; 21: 12174-180.

12. Carson F.L, Histotechnology 1997; Chicago, IL: ASCP Press;

13. Noy Y, Sklan D, Digestion and absorbtion in the young chickPoultry Science 1995; 74: 366-373. http://dx.doi.org/10.3382/ps.0740366 PMid: 7724461

14. Kocan A.A, Crawford J.A, An ostrich farmers handbook 1999; New York: CAB International; PMCid: PMC96283

15. Takata K, Glucose transporters in the transepithelial transport of glucoseJ. Electron Microsc 1996; 45: 4275-284. http://dx.doi.org/10.1093/oxfordjournals.jmicro.a023443

16. Uldry M, Ibberson M, Hosokawa M, Thorens B, GLUT2 is a high affinity glucosamine transporterFEBS Lett 2002; 524: 1-3199-203. http://dx.doi.org/10.1016/S0014-5793(02)03058-2

17. Ferraris R.P, Dietary and developmental regulation of intestinal sugar transportBiochem. J 2001; 360: 265-276. http://dx.doi.org/10.1042/bj3600265 PMid: 11716754 PMCid: PMC1222226

18. Uldry M, Thorens B, The SLC2 family of facilitated hexose and polyol transportersPflügers Arch 2004; 447: 480-489. http://dx.doi.org/10.1007/s00424-003-1085-0 PMid: 12750891

19. Barone S, Fussell S.L, Singh A.K, S1c2a5 (Glut5) is essential for the absorption of fructose in the intestine and generation of fructose-induced hypertensionJ Biol Chem 2009; 284: 85056-5066. http://dx.doi.org/10.1074/jbc.M808128200 PMid: 19091748 PMCid: PMC2643499

20. Kellett G.L, Brot-Laroche E, Apical GLUT2: a major pathway of intestinal sugar absorptionDiabetes 2005; 54: 103056-3062. http://dx.doi.org/10.2337/diabetes.54.10.3056 PMid: 16186415

Copyright

© 2016 Hussar P. This is an open-access article publishedunder the terms of the Creative Commons Attribution License whichpermits unrestricted use, distribution, and reproduction in any medium,provided the original author and source are credited.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 39, Issue 2, Pages 225-231, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.1515/macvetrev-2016-0089, 2016