Original Scientific Article
Effect of silver nanoparticles on healing of third-degree burns infected with Pseudomonas aeruginosa in laboratory mice
Mukhallad Abdul Kareem Ramadhan*,
Abbas Najee Balasm,
Sanaa Basheer Kadhem,
Haider Faleh Al-Saedi

Mac Vet Rev 2021; 44 (1): 17 - 28


Received: 16 February 2020

Received in revised form: 24 October 2020

Accepted: 06 November 2020

Available Online First: 11 December 2020

Published on: 15 March 2021

Correspondence: Mukhallad Abdul Kareem Ramadhan, mukalad.mcm@uomisan.edu.iq


The treatment of full-thickness skin burn using nanomaterials is promising as a medical application reducing the risk of infection and severe dermal scarring. Therefore, this study aims to evaluate the effectiveness of nanomaterials, particularly 3% silver nanoparticles containing ointment (3% SNO), on the full-thickness skin burn of laboratory mice. A total number of 36 male mice were used, equally divided into three groups: negative control (not burned and not treated); positive control (+ve) (burned and treated with castor oil and white petroleum jelly); and SNO-treated group (burned and treated with 3% SNO). The skin of the animals’ back was shaved. A 2x0.5 cm metal plate was heated on a burner to burn the skin of the animals of positive control and SNO-treated groups. Pseudomonas aeruginosa bacterial suspension was applied to the burnt area. The application of SNO, as well as the mixture of white petroleum jelly and castor oil, was started after 6 hours of inducing burns and continued for 14 days (three times daily) in the respected groups. The SNO-treated group showed accelerated healing within 14 days demonstrated by re-epithelialization of the epidermal layer and proliferation of the fibroblasts in the dermal layer. Less healing evidence was observed in the +ve control group in the same period. In conclusion, to our knowledge, this is the first study that uses a 3% SNO formula and has found that it has a promising impact on the treatment of infected skin burns.

Keywords: silver nanoparticles, skin burns, silver ointment, skin healing, infected burns


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© 2020 Ramadhan M.A.K. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 

Conflict of Interest Statement

The authors have declared that no competing interests exist

Citation Information

Macedonian Veterinary Review. Volume 44, Issue 1, Pages 17-28, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2020-0032